40113

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4129368..4129865 Replicon chromosome
Accession NZ_AP025209
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LPH28_RS20095 Protein ID WP_001173343.1
Coordinates 4129368..4129541 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID -
Locus tag LPH28_RS20100 Protein ID WP_071886671.1
Coordinates 4129569..4129865 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS20085 (4126033) 4126033..4127490 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LPH28_RS20090 (4127727) 4127727..4129244 + 1518 WP_001295074.1 lysine--tRNA ligase -
LPH28_RS20095 (4129368) 4129368..4129541 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LPH28_RS20100 (4129569) 4129569..4129865 - 297 WP_071886671.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LPH28_RS20105 (4130092) 4130092..4130364 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LPH28_RS20110 (4130376) 4130376..4130606 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LPH28_RS20115 (4130787) 4130787..4132418 + 1632 WP_001216477.1 sensor histidine kinase -
LPH28_RS20120 (4132415) 4132415..4133134 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4120509..4129865 9356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40113 WP_001173343.1 NZ_AP025209:c4129541-4129368 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40113 NZ_AP025209:c4129541-4129368 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11439.96 Da        Isoelectric Point: 4.1705

>AT40113 WP_071886671.1 NZ_AP025209:c4129865-4129569 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLKSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40113 NZ_AP025209:c4129865-4129569 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAAATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure

References