40111

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3650876..3651417 Replicon chromosome
Accession NZ_AP025209
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0K4UG80
Locus tag LPH28_RS17780 Protein ID WP_033802988.1
Coordinates 3651139..3651417 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A0K5TST2
Locus tag LPH28_RS17775 Protein ID WP_033802987.1
Coordinates 3650876..3651136 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS17755 (3646450) 3646450..3647235 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
LPH28_RS17760 (3647207) 3647207..3648919 + 1713 Protein_3477 flagellar biosynthesis protein FlhA -
LPH28_RS17765 (3649244) 3649244..3649741 - 498 WP_033802985.1 REP-associated tyrosine transposase RayT -
LPH28_RS17770 (3649917) 3649917..3650666 - 750 WP_033802986.1 C40 family peptidase -
LPH28_RS17775 (3650876) 3650876..3651136 + 261 WP_033802987.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LPH28_RS17780 (3651139) 3651139..3651417 + 279 WP_033802988.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LPH28_RS17785 (3651573) 3651573..3652313 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LPH28_RS17790 (3652284) 3652284..3653051 - 768 WP_000333380.1 class II glutamine amidotransferase -
LPH28_RS17795 (3653257) 3653257..3653835 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10828.65 Da        Isoelectric Point: 10.0702

>T40111 WP_033802988.1 NZ_AP025209:3651139-3651417 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAVLFG

Download         Length: 279 bp

>T40111 NZ_AP025209:3651139-3651417 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATAAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTACAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGTGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT40111 WP_033802987.1 NZ_AP025209:3650876-3651136 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKNADDL
FDKLGI

Download         Length: 261 bp

>AT40111 NZ_AP025209:3650876-3651136 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAAACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UG80

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5TST2

References