Detailed information of TA system TA040110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3644578..3645272
• Replicon: chromosome
• Accession: NZ_AP025209
• Organism: Escherichia coli strain 2017.06.04CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LPH28_RS17740
• Protein ID: WP_001263493.1
• Coordinates: 3644578..3644976 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LPH28_RS17745
• Protein ID: WP_000554757.1
• Coordinates: 3644979..3645272 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3640238)	3640238..3640318	-	81	NuclAT_9	-	-
- (3640238)	3640238..3640318	-	81	NuclAT_9	-	-
- (3640238)	3640238..3640318	-	81	NuclAT_9	-	-
- (3640238)	3640238..3640318	-	81	NuclAT_9	-	-
LPH28_RS17710 (3639578)	3639578..3640822	-	1245	WP_000189541.1	esterase FrsA	-
LPH28_RS17715 (3640914)	3640914..3641372	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LPH28_RS17720 (3641633)	3641633..3643090	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
LPH28_RS17725 (3643147)	3643147..3643668	-	522	Protein_3470	peptide chain release factor H	-
LPH28_RS17730 (3643667)	3643667..3643870	-	204	Protein_3471	RtcB family protein	-
LPH28_RS17735 (3644116)	3644116..3644568	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LPH28_RS17740 (3644578)	3644578..3644976	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LPH28_RS17745 (3644979)	3644979..3645272	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LPH28_RS17750 (3645324)	3645324..3646379	-	1056	WP_001226164.1	DNA polymerase IV	-
LPH28_RS17755 (3646450)	3646450..3647235	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
LPH28_RS17760 (3647207)	3647207..3648919	+	1713	Protein_3477	flagellar biosynthesis protein FlhA	-
LPH28_RS17765 (3649244)	3649244..3649741	-	498	WP_033802985.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T40110 WP_001263493.1 NZ_AP025209:c3644976-3644578 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40110 NZ_AP025209:c3644976-3644578 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT40110 WP_000554757.1 NZ_AP025209:c3645272-3644979 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40110 NZ_AP025209:c3645272-3644979 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6