Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1746118..1746620 Replicon chromosome
Accession NZ_AP025209
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LPH28_RS08260 Protein ID WP_000767829.1
Coordinates 1746366..1746620 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LPH28_RS08255 Protein ID WP_001259255.1
Coordinates 1746118..1746369 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS08230 (1741296) 1741296..1742363 - 1068 WP_000080118.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LPH28_RS08235 (1742363) 1742363..1743433 - 1071 WP_109544655.1 histidinol-phosphate transaminase -
LPH28_RS08240 (1743430) 1743430..1744734 - 1305 WP_001300981.1 histidinol dehydrogenase -
LPH28_RS08245 (1744740) 1744740..1745639 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
LPH28_RS08250 (1745785) 1745785..1745835 - 51 WP_001373527.1 his operon leader peptide -
LPH28_RS08255 (1746118) 1746118..1746369 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LPH28_RS08260 (1746366) 1746366..1746620 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LPH28_RS08265 (1746703) 1746703..1747527 + 825 WP_109544656.1 SDR family oxidoreductase -
LPH28_RS08270 (1747573) 1747573..1748502 + 930 WP_001374016.1 LysR substrate-binding domain-containing protein -
LPH28_RS08275 (1748717) 1748717..1748779 + 63 WP_010723108.1 membrane protein YoeI -
LPH28_RS08280 (1748769) 1748769..1750127 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T40096 WP_000767829.1 NZ_AP025209:1746366-1746620 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T40096 NZ_AP025209:1746366-1746620 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT40096 WP_001259255.1 NZ_AP025209:1746118-1746369 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT40096 NZ_AP025209:1746118-1746369 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References