Detailed information of TA system TA040084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3575572..3576266
• Replicon: chromosome
• Accession: NZ_AP025205
• Organism: Escherichia coli strain 2017.04.03CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LPH27_RS17215
• Protein ID: WP_001263493.1
• Coordinates: 3575572..3575970 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LPH27_RS17220
• Protein ID: WP_000554757.1
• Coordinates: 3575973..3576266 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3571232)	3571232..3571312	-	81	NuclAT_9	-	-
- (3571232)	3571232..3571312	-	81	NuclAT_9	-	-
- (3571232)	3571232..3571312	-	81	NuclAT_9	-	-
- (3571232)	3571232..3571312	-	81	NuclAT_9	-	-
LPH27_RS17185 (3570572)	3570572..3571816	-	1245	WP_000189541.1	esterase FrsA	-
LPH27_RS17190 (3571908)	3571908..3572366	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LPH27_RS17195 (3572627)	3572627..3574084	+	1458	WP_229266163.1	cytosol nonspecific dipeptidase	-
LPH27_RS17200 (3574141)	3574141..3574662	-	522	Protein_3362	peptide chain release factor H	-
LPH27_RS17205 (3574661)	3574661..3574864	-	204	Protein_3363	RtcB family protein	-
LPH27_RS17210 (3575110)	3575110..3575562	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LPH27_RS17215 (3575572)	3575572..3575970	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LPH27_RS17220 (3575973)	3575973..3576266	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LPH27_RS17225 (3576318)	3576318..3577373	-	1056	WP_001226164.1	DNA polymerase IV	-
LPH27_RS17230 (3577444)	3577444..3578229	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
LPH27_RS17235 (3578201)	3578201..3579913	+	1713	Protein_3369	flagellar biosynthesis protein FlhA	-
LPH27_RS17240 (3580137)	3580137..3580634	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T40084 WP_001263493.1 NZ_AP025205:c3575970-3575572 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40084 NZ_AP025205:c3575970-3575572 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT40084 WP_000554757.1 NZ_AP025205:c3576266-3575973 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40084 NZ_AP025205:c3576266-3575973 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6