Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1790482..1790984 Replicon chromosome
Accession NZ_AP025205
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag LPH27_RS08470 Protein ID WP_000767833.1
Coordinates 1790730..1790984 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LPH27_RS08465 Protein ID WP_001259255.1
Coordinates 1790482..1790733 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS08440 (1785661) 1785661..1786728 - 1068 WP_032202492.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LPH27_RS08445 (1786728) 1786728..1787798 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
LPH27_RS08450 (1787795) 1787795..1789099 - 1305 WP_058905757.1 histidinol dehydrogenase -
LPH27_RS08455 (1789105) 1789105..1790004 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
LPH27_RS08460 (1790150) 1790150..1790200 - 51 WP_001364200.1 his operon leader peptide -
LPH27_RS08465 (1790482) 1790482..1790733 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LPH27_RS08470 (1790730) 1790730..1790984 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LPH27_RS08475 (1791067) 1791067..1791891 + 825 WP_032202495.1 SDR family oxidoreductase -
LPH27_RS08480 (1791937) 1791937..1792866 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LPH27_RS08485 (1793081) 1793081..1793143 + 63 WP_010723108.1 membrane protein YoeI -
LPH27_RS08490 (1793133) 1793133..1794491 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LPH27_RS08495 (1794670) 1794670..1795728 + 1059 WP_000492312.1 thiosulfate utilization transporter TsuA/YeeE -
LPH27_RS08500 (1795742) 1795742..1795969 + 228 WP_000985278.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T40070 WP_000767833.1 NZ_AP025205:1790730-1790984 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T40070 NZ_AP025205:1790730-1790984 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT40070 WP_001259255.1 NZ_AP025205:1790482-1790733 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT40070 NZ_AP025205:1790482-1790733 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References