Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 741934..742627 Replicon chromosome
Accession NZ_AP025205
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag LPH27_RS03580 Protein ID WP_000415584.1
Coordinates 741934..742230 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag LPH27_RS03585 Protein ID WP_000650107.1
Coordinates 742232..742627 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS03545 (737022) 737022..737336 - 315 WP_000958598.1 putative quinol monooxygenase -
LPH27_RS03550 (737367) 737367..737948 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
LPH27_RS03555 (738267) 738267..738599 + 333 WP_000917684.1 DUF2645 family protein -
LPH27_RS03560 (738645) 738645..739994 - 1350 WP_001618857.1 quorum sensing histidine kinase QseC -
LPH27_RS03565 (739991) 739991..740650 - 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB -
LPH27_RS03570 (740802) 740802..741194 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
LPH27_RS03575 (741247) 741247..741729 + 483 WP_000183505.1 GyrI-like domain-containing protein -
LPH27_RS03580 (741934) 741934..742230 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
LPH27_RS03585 (742232) 742232..742627 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
LPH27_RS03590 (742760) 742760..744367 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
LPH27_RS03595 (744505) 744505..746763 + 2259 WP_032161229.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T40067 WP_000415584.1 NZ_AP025205:741934-742230 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T40067 NZ_AP025205:741934-742230 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT40067 WP_000650107.1 NZ_AP025205:742232-742627 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT40067 NZ_AP025205:742232-742627 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References