Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4795831..4796333 Replicon chromosome
Accession NZ_AP025180
Organism Escherichia coli strain JP538

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag R5G_RS23530 Protein ID WP_000767828.1
Coordinates 4796079..4796333 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag R5G_RS23525 Protein ID WP_001259255.1
Coordinates 4795831..4796082 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R5G_RS23500 (4791009) 4791009..4792076 - 1068 WP_096216168.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
R5G_RS23505 (4792076) 4792076..4793146 - 1071 WP_085442794.1 histidinol-phosphate transaminase -
R5G_RS23510 (4793143) 4793143..4794447 - 1305 WP_001350335.1 histidinol dehydrogenase -
R5G_RS23515 (4794453) 4794453..4795352 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
R5G_RS23520 (4795498) 4795498..4795548 - 51 WP_001364200.1 his operon leader peptide -
R5G_RS23525 (4795831) 4795831..4796082 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
R5G_RS23530 (4796079) 4796079..4796333 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
R5G_RS23535 (4796416) 4796416..4797240 + 825 WP_000754740.1 SDR family oxidoreductase -
R5G_RS23540 (4797286) 4797286..4798215 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
R5G_RS23545 (4798430) 4798430..4798492 + 63 WP_010723108.1 membrane protein YoeI -
R5G_RS23550 (4798482) 4798482..4799840 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
R5G_RS23555 (4800019) 4800019..4801077 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
R5G_RS23560 (4801091) 4801091..4801318 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T40052 WP_000767828.1 NZ_AP025180:4796079-4796333 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T40052 NZ_AP025180:4796079-4796333 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT40052 WP_001259255.1 NZ_AP025180:4795831-4796082 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT40052 NZ_AP025180:4795831-4796082 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References