Detailed information of TA system TA040050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3878706..3879400
• Replicon: chromosome
• Accession: NZ_AP025180
• Organism: Escherichia coli strain JP538

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: R5G_RS19055
• Protein ID: WP_001263489.1
• Coordinates: 3879002..3879400 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: R5G_RS19050
• Protein ID: WP_096216098.1
• Coordinates: 3878706..3878999 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R5G_RS19030 (3874339)	3874339..3874836	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
R5G_RS19035 (3875060)	3875060..3876772	-	1713	Protein_3731	flagellar biosynthesis protein FlhA	-
R5G_RS19040 (3876744)	3876744..3877528	+	785	Protein_3732	putative lateral flagellar export/assembly protein LafU	-
R5G_RS19045 (3877599)	3877599..3878654	+	1056	WP_001226164.1	DNA polymerase IV	-
R5G_RS19050 (3878706)	3878706..3878999	+	294	WP_096216098.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
R5G_RS19055 (3879002)	3879002..3879400	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
R5G_RS19060 (3879410)	3879410..3879862	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
R5G_RS19065 (3880180)	3880180..3880386	+	207	Protein_3737	RtcB family protein	-
R5G_RS19070 (3880382)	3880382..3880903	+	522	Protein_3738	peptide chain release factor H	-
R5G_RS19075 (3880960)	3880960..3882417	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
R5G_RS19080 (3882678)	3882678..3883136	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3883732)	3883732..3883812	+	81	NuclAT_12	-	-
- (3883732)	3883732..3883812	+	81	NuclAT_12	-	-
- (3883732)	3883732..3883812	+	81	NuclAT_12	-	-
- (3883732)	3883732..3883812	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	rhs/PAAR / gmhA/lpcA	3858181..3880903	22722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T40050 WP_001263489.1 NZ_AP025180:3879002-3879400 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40050 NZ_AP025180:3879002-3879400 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11180.71 Da
• Isoelectric Point: 4.9059

>AT40050 WP_096216098.1 NZ_AP025180:3878706-3878999 [Escherichia coli]
MHRILAEKSVNITELCKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40050 NZ_AP025180:3878706-3878999 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTATGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1