Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3872663..3873204 Replicon chromosome
Accession NZ_AP025180
Organism Escherichia coli strain JP538

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag R5G_RS19015 Protein ID WP_000615983.1
Coordinates 3872663..3872941 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag R5G_RS19020 Protein ID WP_000729703.1
Coordinates 3872944..3873204 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R5G_RS19000 (3870245) 3870245..3870823 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
R5G_RS19005 (3871029) 3871029..3871796 + 768 WP_000333380.1 class II glutamine amidotransferase -
R5G_RS19010 (3871767) 3871767..3872507 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
R5G_RS19015 (3872663) 3872663..3872941 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
R5G_RS19020 (3872944) 3872944..3873204 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
R5G_RS19025 (3873414) 3873414..3874163 + 750 WP_000056849.1 C40 family peptidase -
R5G_RS19030 (3874339) 3874339..3874836 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
R5G_RS19035 (3875060) 3875060..3876772 - 1713 Protein_3731 flagellar biosynthesis protein FlhA -
R5G_RS19040 (3876744) 3876744..3877528 + 785 Protein_3732 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 3858181..3880903 22722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T40049 WP_000615983.1 NZ_AP025180:c3872941-3872663 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T40049 NZ_AP025180:c3872941-3872663 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT40049 WP_000729703.1 NZ_AP025180:c3873204-3872944 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT40049 NZ_AP025180:c3873204-3872944 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References