Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2127773..2128500 Replicon chromosome
Accession NZ_AP025180
Organism Escherichia coli strain JP538

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag R5G_RS10590 Protein ID WP_000550189.1
Coordinates 2128186..2128500 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag R5G_RS10585 Protein ID WP_000560266.1
Coordinates 2127773..2128189 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R5G_RS10575 (2122933) 2122933..2125284 + 2352 WP_000695487.1 alpha-glucosidase -
R5G_RS10580 (2125710) 2125710..2127728 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
R5G_RS10585 (2127773) 2127773..2128189 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
R5G_RS10590 (2128186) 2128186..2128500 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
R5G_RS10595 (2128784) 2128784..2129920 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
R5G_RS10600 (2130005) 2130005..2130508 + 504 WP_001295542.1 M48 family metallopeptidase -
R5G_RS10605 (2130585) 2130585..2130659 + 75 Protein_2093 vancomycin high temperature exclusion protein -
R5G_RS10610 (2130691) 2130691..2132262 - 1572 WP_000381442.1 IS66 family transposase -
R5G_RS10615 (2132282) 2132282..2132629 - 348 WP_000624618.1 IS66 family insertion sequence element accessory protein TnpB -
R5G_RS10620 (2132629) 2132629..2133279 - 651 WP_000993956.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T40045 WP_000550189.1 NZ_AP025180:c2128500-2128186 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T40045 NZ_AP025180:c2128500-2128186 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT40045 WP_000560266.1 NZ_AP025180:c2128189-2127773 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT40045 NZ_AP025180:c2128189-2127773 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References