Detailed information of TA system TA039999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2487838..2488355
• Replicon: chromosome
• Accession: NZ_AP025176
• Organism: Staphylococcus aureus strain TPS5614

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LPC25_RS12470
• Protein ID: WP_000113261.1
• Coordinates: 2487838..2488104 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: LPC25_RS12475
• Protein ID: WP_000584499.1
• Coordinates: 2488104..2488355 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPC25_RS12440 (2483275)	2483275..2483811	-	537	WP_000169319.1	GNAT family N-acetyltransferase	-
LPC25_RS12445 (2484045)	2484045..2484869	-	825	WP_000594640.1	formate/nitrite transporter family protein	-
LPC25_RS12450 (2485072)	2485072..2485254	-	183	WP_000250735.1	hypothetical protein	-
LPC25_RS12455 (2485341)	2485341..2485808	-	468	WP_000153345.1	SRPBCC domain-containing protein	-
LPC25_RS12460 (2485994)	2485994..2487544	-	1551	WP_000727760.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LPC25_RS12465 (2487732)	2487732..2487803	-	72	WP_223200558.1	hypothetical protein	-
LPC25_RS12470 (2487838)	2487838..2488104	-	267	WP_000113261.1	Txe/YoeB family addiction module toxin	Toxin
LPC25_RS12475 (2488104)	2488104..2488355	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPC25_RS12480 (2488518)	2488518..2488619	-	102	Protein_2420	hypothetical protein	-
LPC25_RS12485 (2488626)	2488626..2489225	-	600	WP_000162810.1	DsbA family protein	-
LPC25_RS12490 (2489244)	2489244..2489606	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
LPC25_RS12495 (2489861)	2489861..2491111	+	1251	WP_001012231.1	FemA/FemB family glycyltransferase FmhA	-
LPC25_RS12500 (2491196)	2491196..2491927	-	732	WP_000615455.1	amino acid ABC transporter ATP-binding protein	-
LPC25_RS12505 (2491924)	2491924..2492643	-	720	WP_000479573.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10452.02 Da
• Isoelectric Point: 10.0373

>T39999 WP_000113261.1 NZ_AP025176:c2488104-2487838 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDKHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T39999 NZ_AP025176:c2488104-2487838 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAAGCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT39999 WP_000584499.1 NZ_AP025176:c2488355-2488104 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT39999 NZ_AP025176:c2488355-2488104 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAGCACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: T1YB60