Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1843916..1844523 Replicon chromosome
Accession NZ_AP025169
Organism Pseudomonas sp. CYM-20-01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag M2497_RS08210 Protein ID WP_248488179.1
Coordinates 1843916..1844230 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M2497_RS08215 Protein ID WP_248488180.1
Coordinates 1844230..1844523 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2497_RS08180 (cym2001_16120) 1839035..1839535 - 501 WP_248486781.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
M2497_RS08185 (cym2001_16130) 1839626..1840036 - 411 WP_071496822.1 type II toxin-antitoxin system HicB family antitoxin -
M2497_RS08190 (cym2001_16140) 1840059..1840241 - 183 WP_248486783.1 type II toxin-antitoxin system HicA family toxin -
M2497_RS08195 (cym2001_16150) 1840410..1841303 - 894 WP_248486785.1 LysR family transcriptional regulator -
M2497_RS08200 (cym2001_16160) 1841399..1842562 + 1164 WP_248486788.1 MFS transporter -
M2497_RS08205 (cym2001_16170) 1842559..1843728 + 1170 WP_248486790.1 MFS transporter -
M2497_RS08210 1843916..1844230 + 315 WP_248488179.1 hypothetical protein Toxin
M2497_RS08215 (cym2001_16180) 1844230..1844523 + 294 WP_248488180.1 helix-turn-helix domain-containing protein Antitoxin
M2497_RS08225 (cym2001_16190) 1845193..1846077 - 885 WP_248486792.1 alpha/beta hydrolase -
M2497_RS08230 (cym2001_16200) 1846383..1847075 - 693 WP_248486794.1 pseudouridine synthase -
M2497_RS08235 (cym2001_16210) 1847075..1847287 - 213 WP_071488921.1 cysteine-rich CWC family protein -
M2497_RS08240 (cym2001_16220) 1847361..1848983 - 1623 WP_248486796.1 methyl-accepting chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11879.67 Da        Isoelectric Point: 9.4400

>T39974 WP_248488179.1 NZ_AP025169:1843916-1844230 [Pseudomonas sp. CYM-20-01]
MIFIESPAFTEDLLGLLSEESYGEFQAFLAENPTSGDVIQGTNGLRKIRWAVRGRGKSGGVRVIYYHVCKAYQIRLILIY
RKGIKDDLSLAQRSVLSEINRRWE

Download         Length: 315 bp

>T39974 NZ_AP025169:1843916-1844230 [Pseudomonas sp. CYM-20-01]
ATGATATTTATCGAGTCACCCGCATTTACAGAAGATCTGCTAGGGCTTCTGAGTGAGGAAAGCTACGGTGAATTCCAAGC
GTTTTTGGCCGAAAATCCCACCTCCGGTGATGTCATTCAAGGCACCAATGGCTTGAGAAAAATCCGGTGGGCCGTGCGGG
GAAGGGGAAAGAGCGGAGGCGTCAGAGTGATTTATTATCATGTCTGCAAGGCCTATCAAATCAGGTTGATCTTGATCTAC
CGGAAGGGCATTAAAGATGACCTTTCGCTGGCGCAGAGAAGTGTTCTGAGTGAAATAAACAGAAGGTGGGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10940.55 Da        Isoelectric Point: 8.8445

>AT39974 WP_248488180.1 NZ_AP025169:1844230-1844523 [Pseudomonas sp. CYM-20-01]
MEKKLFNRLVESLIQMDEIVSGERPPSREFFVDALKVKEIRRATGLSQAGFAKAIDVPVGTLQNWEQGRREPEGPARALL
RALHNDPSHVLAALEHR

Download         Length: 294 bp

>AT39974 NZ_AP025169:1844230-1844523 [Pseudomonas sp. CYM-20-01]
ATGGAGAAAAAACTTTTCAACCGTCTGGTCGAAAGTTTGATTCAGATGGATGAGATCGTAAGTGGCGAGCGACCTCCTTC
TCGCGAATTTTTTGTTGATGCGTTGAAGGTAAAAGAGATTCGACGAGCCACCGGGCTTTCCCAAGCGGGCTTTGCAAAGG
CGATTGACGTGCCTGTAGGAACACTTCAGAACTGGGAGCAAGGGCGACGTGAGCCAGAAGGGCCGGCTAGAGCATTGCTT
CGTGCCTTACACAACGACCCTAGTCACGTATTGGCAGCGCTGGAGCATCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References