Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 1293274..1293871 Replicon chromosome
Accession NZ_AP025160
Organism Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag ANFP_RS06645 Protein ID WP_054608787.1
Coordinates 1293274..1293552 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ANFP_RS06650 Protein ID WP_054608786.1
Coordinates 1293569..1293871 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ANFP_RS06625 (ANFP_13330) 1288352..1290427 - 2076 WP_157293188.1 zincin-like metallopeptidase domain-containing protein -
ANFP_RS06630 (ANFP_13340) 1290408..1290587 - 180 WP_146235814.1 hypothetical protein -
ANFP_RS06635 (ANFP_13350) 1290727..1291662 - 936 WP_070807680.1 DNA adenine methylase -
ANFP_RS06640 (ANFP_13360) 1291666..1293117 - 1452 WP_054608788.1 toprim domain-containing protein -
ANFP_RS06645 (ANFP_13370) 1293274..1293552 + 279 WP_054608787.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ANFP_RS06650 (ANFP_13380) 1293569..1293871 + 303 WP_054608786.1 HigA family addiction module antitoxin Antitoxin
ANFP_RS06655 (ANFP_13390) 1293890..1295827 - 1938 WP_054608785.1 relaxase/mobilization nuclease domain-containing protein -
ANFP_RS06660 (ANFP_13400) 1295827..1296153 - 327 WP_054608784.1 hypothetical protein -
ANFP_RS06665 (ANFP_13410) 1296321..1296593 + 273 WP_054608783.1 HU family DNA-binding protein -
ANFP_RS06670 1296620..1296886 + 267 WP_054608782.1 superinfection immunity protein -
ANFP_RS06675 (ANFP_13430) 1296957..1297379 + 423 WP_054608781.1 hypothetical protein -
ANFP_RS06680 (ANFP_13440) 1297498..1298658 - 1161 WP_229129786.1 cellulose synthase complex periplasmic endoglucanase BcsZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-92)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10745.15 Da        Isoelectric Point: 7.8253

>T39959 WP_054608787.1 NZ_AP025160:1293274-1293552 [Acidithiobacillus ferrooxidans]
MIKSFRCKDTQSLFEGGAPRRFQGVRPQAERKLLMVDSAKDLKDLKSPPGNGLEKLSGDRKDQWSIRINDQWRICFDWRD
GDAYDVEIVDYH

Download         Length: 279 bp

>T39959 NZ_AP025160:1293274-1293552 [Acidithiobacillus ferrooxidans]
ATGATCAAGTCTTTCCGTTGCAAGGATACGCAAAGCCTGTTTGAAGGCGGGGCACCGCGCCGATTTCAGGGGGTCAGGCC
CCAAGCCGAGCGGAAGCTGTTGATGGTGGATTCGGCGAAGGATCTGAAGGACTTGAAAAGCCCGCCGGGTAACGGCTTAG
AGAAGCTCTCCGGAGACCGCAAAGATCAGTGGAGCATTCGTATCAACGACCAGTGGCGAATATGCTTCGATTGGCGAGAT
GGCGATGCGTATGACGTTGAAATCGTGGATTACCATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11439.21 Da        Isoelectric Point: 10.8303

>AT39959 WP_054608786.1 NZ_AP025160:1293569-1293871 [Acidithiobacillus ferrooxidans]
MTRNRMRPIHPGEVLREEFLAPLGMTPHALAVALRVPSPRINDITRERRAITPDTALRLARYFGTTPEFWMELQSVYDLR
RAEVIAGESISREVTPRQAA

Download         Length: 303 bp

>AT39959 NZ_AP025160:1293569-1293871 [Acidithiobacillus ferrooxidans]
ATGACTAGAAACAGGATGCGCCCGATTCACCCTGGCGAAGTGTTGCGCGAGGAGTTTTTGGCCCCGCTGGGTATGACGCC
CCATGCGTTGGCGGTCGCGTTGCGCGTACCATCACCGCGCATCAATGACATAACGCGGGAACGGCGGGCGATTACGCCGG
ATACTGCCTTGCGGCTGGCCCGCTATTTCGGGACGACCCCGGAATTCTGGATGGAACTGCAGAGTGTTTATGACCTGCGC
AGGGCTGAGGTTATCGCCGGTGAGTCTATCTCTCGTGAGGTGACCCCGCGGCAGGCGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References