Detailed information of TA system TA039955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 87242..87850
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W1K1W3
• Locus tag: ANFP_RS00440
• Protein ID: WP_012535782.1
• Coordinates: 87242..87517 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ANFP_RS00445
• Protein ID: WP_009566298.1
• Coordinates: 87533..87850 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS00405 (ANFP_00830)	83020..84060	+	1041	WP_012535779.1	glycosyltransferase family 1 protein	-
ANFP_RS00410 (ANFP_00840)	84128..85054	+	927	WP_012535780.1	glycosyltransferase	-
ANFP_RS00415 (ANFP_00850)	85047..85415	+	369	WP_012535781.1	glycosyltransferase	-
ANFP_RS00425 (ANFP_00860)	86155..86376	+	222	WP_009566302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ANFP_RS00430 (ANFP_00870)	86376..86789	+	414	WP_009566301.1	type II toxin-antitoxin system VapC family toxin	-
ANFP_RS00435 (ANFP_00880)	86786..87076	+	291	WP_009566300.1	hypothetical protein	-
ANFP_RS00440 (ANFP_00890)	87242..87517	+	276	WP_012535782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANFP_RS00445 (ANFP_00900)	87533..87850	+	318	WP_009566298.1	HigA family addiction module antitoxin	Antitoxin
ANFP_RS00450	87865..88044	+	180	WP_009566297.1	HNH endonuclease	-
ANFP_RS16555 (ANFP_00920)	88144..88272	-	129	WP_257246670.1	hypothetical protein	-
ANFP_RS00455 (ANFP_00930)	88464..89627	+	1164	WP_009566294.1	Fic family protein	-
ANFP_RS00460 (ANFP_00940)	89924..90190	-	267	WP_196491178.1	hypothetical protein	-
ANFP_RS00465 (ANFP_00950)	90187..90429	-	243	WP_012535785.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
ANFP_RS00470 (ANFP_00960)	90513..90686	-	174	WP_009562770.1	PCP reductase family protein	-
ANFP_RS00475 (ANFP_00970)	90797..91225	-	429	WP_009562768.1	hypothetical protein	-
ANFP_RS00480 (ANFP_00980)	91228..91659	-	432	WP_009562767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10433.84 Da
• Isoelectric Point: 7.9070

>T39955 WP_012535782.1 NZ_AP025160:87242-87517 [Acidithiobacillus ferrooxidans]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAATSPEELNVPGFDFHALEGKPKRYTVHVNGPYCITFEWREG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T39955 NZ_AP025160:87242-87517 [Acidithiobacillus ferrooxidans]
TTGATACGAAGTTTTGTGCATAAGGGCCTCAAGCAGGTTTTCGAAACAGGTCAATCGGCGAAAGTCGGTGCGGATTTGGT
AACCCGCTGCAAGCGCAGGCTCGATGCCATAAACGCTGCGACATCCCCGGAGGAGTTGAACGTGCCGGGTTTCGACTTCC
ATGCTTTGGAGGGGAAACCGAAGCGGTACACGGTTCATGTGAATGGACCCTATTGCATCACCTTCGAGTGGCGTGAAGGA
GATGCCTGGCGCATTGATCTGGAGAATTACCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11756.54 Da
• Isoelectric Point: 5.6631

>AT39955 WP_009566298.1 NZ_AP025160:87533-87850 [Acidithiobacillus ferrooxidans]
MDEMTVEQVDRIPTHPGEILREEVLPALDISVAEFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELAAIHKPEQAA

Nucleotide

• Length: 318 bp

>AT39955 NZ_AP025160:87533-87850 [Acidithiobacillus ferrooxidans]
ATGGACGAAATGACCGTTGAGCAAGTGGATCGGATACCGACGCATCCTGGCGAGATATTGCGGGAAGAGGTACTGCCCGC
ACTGGATATCTCTGTAGCAGAGTTTGCCCGTGGCCTGCGGGTCTCGCGCCAGACCGTTCACGGCATCCTGTCTGAGCGCA
AGGGCATCACCCCGGAGATGGCCTTGCGTATCGGCAAATATCTGGGTAACGGGCCGGGGATATGGCTACGCATGCAGCAG
GCTTACGATTTGACCGTTGCAGAACGTCGGTTGGCCGAAGAACTGGCGGCCATTCACAAGCCCGAGCAGGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2W1K1W3