Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 232000..232724 Replicon plasmid pNUITM-VR1_2
Accession NZ_AP025011
Organism Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LDO46_RS29870 Protein ID WP_076752146.1
Coordinates 232413..232724 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LDO46_RS29865 Protein ID WP_076752145.1
Coordinates 232000..232416 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO46_RS29835 (NUITMVR1_58880) 227652..227882 + 231 WP_076752135.1 hypothetical protein -
LDO46_RS29840 (NUITMVR1_58890) 228086..228919 - 834 WP_076752137.1 N-6 DNA methylase -
LDO46_RS29845 (NUITMVR1_58900) 228966..229196 - 231 WP_076752138.1 hypothetical protein -
LDO46_RS29850 (NUITMVR1_58910) 229285..229632 - 348 WP_076752140.1 hypothetical protein -
LDO46_RS29855 (NUITMVR1_58920) 229735..230007 - 273 WP_137055850.1 hypothetical protein -
LDO46_RS29865 (NUITMVR1_58950) 232000..232416 - 417 WP_076752145.1 helix-turn-helix domain-containing protein Antitoxin
LDO46_RS29870 (NUITMVR1_58960) 232413..232724 - 312 WP_076752146.1 type II toxin-antitoxin system HigB family toxin Toxin
LDO46_RS29875 (NUITMVR1_58970) 232874..233086 - 213 WP_076752148.1 hypothetical protein -
LDO46_RS29880 (NUITMVR1_58980) 233194..233520 - 327 WP_076752150.1 hypothetical protein -
LDO46_RS29885 234379..234495 - 117 WP_223176086.1 type I toxin-antitoxin system Hok family toxin -
LDO46_RS29890 234443..234595 - 153 WP_223526259.1 DUF5431 family protein -
LDO46_RS29895 (NUITMVR1_59010) 234879..235364 - 486 WP_076752153.1 GNAT family N-acetyltransferase -
LDO46_RS29900 (NUITMVR1_59020) 235352..235618 - 267 WP_012540086.1 DUF1778 domain-containing protein -
LDO46_RS29905 (NUITMVR1_59030) 235919..236242 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
LDO46_RS29910 (NUITMVR1_59040) 236244..236957 + 714 WP_013087170.1 arsenical resistance protein ArsH -
LDO46_RS29915 (NUITMVR1_59050) 236966..237511 + 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 / qnrS1 / aph(6)-Id / aph(3'')-Ib - 1..261835 261835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(5-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12510.31 Da        Isoelectric Point: 9.9242

>T39903 WP_076752146.1 NZ_AP025011:c232724-232413 [Raoultella ornithinolytica]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFNSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp

>T39903 NZ_AP025011:c232724-232413 [Raoultella ornithinolytica]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCAGCGGCCATCGATGACACATA
TCGTGTACTAAAGCGGGTTGTAGCAAAAAAACCGGATGAGCTAAAGCGATACTTTAATAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGCAATAACCTCAGAATGATGTTTTTTGCTGATTTCGAGCGCGGAAAGATA
TTCGTCAAACACATTACCACCCATGCTGAATATGATCGCTTAACGAAATACTATCGGGAGCACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15484.61 Da        Isoelectric Point: 4.4711

>AT39903 WP_076752145.1 NZ_AP025011:c232416-232000 [Raoultella ornithinolytica]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSCERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp

>AT39903 NZ_AP025011:c232416-232000 [Raoultella ornithinolytica]
ATGATTTTTAGCGATGCCATTAAAGCTGCAAATGACCTTGCCAGTATTGTACCCCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGACAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTC
TCATTGCTGCGGGTTCTTATGCAGCAACATGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTTGTGAACGCAGTCTGACCCTTGATCACATGCGAGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References