Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 350479..351094 Replicon chromosome
Accession NZ_AP024968
Organism Vibrio cholerae strain V130003

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag M2Z51_RS15700 Protein ID WP_032477340.1
Coordinates 350479..350766 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M2Z51_RS15705 Protein ID WP_032477342.1
Coordinates 350777..351094 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2Z51_RS15655 345805..345996 + 192 WP_113707167.1 disulfide bond formation protein DsbD -
M2Z51_RS15660 346014..346250 + 237 WP_032477346.1 DUF3709 domain-containing protein -
M2Z51_RS15665 346641..346823 + 183 WP_032477360.1 DUF645 family protein -
M2Z51_RS15670 (V130003_30520) 347475..347795 + 321 WP_000248687.1 DUF4144 domain-containing protein -
M2Z51_RS15675 (V130003_30530) 347962..348321 + 360 WP_001071517.1 VOC family protein -
M2Z51_RS15680 348305..348463 + 159 WP_248529924.1 DUF3265 domain-containing protein -
M2Z51_RS15685 (V130003_30540) 348460..348846 + 387 WP_029628680.1 MmcQ/YjbR family DNA-binding protein -
M2Z51_RS15690 (V130003_30550) 348988..349347 + 360 WP_001900224.1 hypothetical protein -
M2Z51_RS15695 (V130003_30560) 349497..349793 + 297 WP_001069046.1 hypothetical protein -
M2Z51_RS15700 (V130003_30570) 350479..350766 + 288 WP_032477340.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2Z51_RS15705 (V130003_30580) 350777..351094 + 318 WP_032477342.1 HigA family addiction module antitoxin Antitoxin
M2Z51_RS15710 (V130003_30590) 351295..351576 + 282 WP_032477343.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M2Z51_RS15715 (V130003_30600) 351573..351860 + 288 WP_000869999.1 type II toxin-antitoxin system RelE/ParE family toxin -
M2Z51_RS15720 (V130003_30610) 352015..352512 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
M2Z51_RS15725 (V130003_30620) 352509..352781 - 273 WP_000246253.1 DUF1778 domain-containing protein -
M2Z51_RS15730 353170..353310 + 141 WP_001952202.1 DUF645 family protein -
M2Z51_RS15735 (V130003_30630) 353557..353895 + 339 WP_050503336.1 hypothetical protein -
M2Z51_RS15740 (V130003_30640) 354062..354598 + 537 WP_000469478.1 GNAT family protein -
M2Z51_RS15745 (V130003_30650) 354769..355131 + 363 WP_000391481.1 DUF6232 family protein -
M2Z51_RS15750 (V130003_30660) 355318..355554 - 237 WP_000772125.1 RNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 304895..371259 66364
- inside Integron - - 305566..368714 63148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T39878 WP_032477340.1 NZ_AP024968:350479-350766 [Vibrio cholerae]
MTLEFKDKWLEQFYEDDKRHRLIPGSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T39878 NZ_AP024968:350479-350766 [Vibrio cholerae]
ATGACATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAGGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11732.70 Da        Isoelectric Point: 10.6985

>AT39878 WP_032477342.1 NZ_AP024968:350777-351094 [Vibrio cholerae]
MRKTKRRPVSVGQMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEVKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT39878 NZ_AP024968:350777-351094 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGCAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGTGTACACAGAAATACGGTGAGTAATTTGATTAATGGCGGAGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAATACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGTAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References