Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-MqsA |
| Location | 322354..322942 | Replicon | chromosome |
| Accession | NZ_AP024968 | ||
| Organism | Vibrio cholerae strain V130003 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | M2Z51_RS15410 | Protein ID | WP_032477326.1 |
| Coordinates | 322354..322641 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | M2Z51_RS15415 | Protein ID | WP_000071008.1 |
| Coordinates | 322628..322942 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M2Z51_RS15355 (V130003_30120) | 317533..317829 | + | 297 | WP_001069046.1 | hypothetical protein | - |
| M2Z51_RS15360 | 318581..318799 | + | 219 | WP_001973795.1 | DUF3709 domain-containing protein | - |
| M2Z51_RS15370 | 319335..319424 | + | 90 | WP_235188938.1 | Tfp pilus assembly protein | - |
| M2Z51_RS15375 (V130003_30130) | 319586..320041 | + | 456 | Protein_273 | GNAT family N-acetyltransferase | - |
| M2Z51_RS15380 (V130003_30140) | 320182..320478 | + | 297 | WP_000617998.1 | Dabb family protein | - |
| M2Z51_RS15385 (V130003_30150) | 320632..321099 | + | 468 | WP_050503362.1 | hypothetical protein | - |
| M2Z51_RS15390 | 321402..321524 | + | 123 | WP_080284773.1 | DUF645 family protein | - |
| M2Z51_RS15395 | 321702..321860 | + | 159 | Protein_277 | acetyltransferase | - |
| M2Z51_RS15400 | 321964..322117 | + | 154 | Protein_278 | DUF645 family protein | - |
| M2Z51_RS15405 | 322234..322296 | + | 63 | Protein_279 | acetyltransferase | - |
| M2Z51_RS15410 (V130003_30160) | 322354..322641 | + | 288 | WP_032477326.1 | toxin HigB-2 | Toxin |
| M2Z51_RS15415 (V130003_30170) | 322628..322942 | + | 315 | WP_000071008.1 | DNA-binding transcriptional regulator | Antitoxin |
| M2Z51_RS15420 (V130003_30180) | 323111..323614 | + | 504 | WP_032477327.1 | DinB family protein | - |
| M2Z51_RS15425 (V130003_30190) | 323798..324028 | - | 231 | WP_000838533.1 | PAS factor family protein | - |
| M2Z51_RS15430 (V130003_30210) | 324256..324795 | + | 540 | WP_000884557.1 | hydrolase | - |
| M2Z51_RS15435 | 325105..325287 | + | 183 | WP_000923320.1 | DUF645 family protein | - |
| M2Z51_RS15440 (V130003_30220) | 325486..325800 | + | 315 | WP_000248689.1 | DUF4144 domain-containing protein | - |
| M2Z51_RS15445 | 326145..326291 | + | 147 | WP_228006404.1 | DUF645 family protein | - |
| M2Z51_RS15450 (V130003_30230) | 326509..326865 | + | 357 | WP_001094969.1 | DUF6404 family protein | - |
| M2Z51_RS15455 (V130003_30240) | 327009..327326 | - | 318 | WP_001180238.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| M2Z51_RS15460 (V130003_30250) | 327345..327587 | - | 243 | WP_000557291.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | qnrVC4 | - | 304895..371259 | 66364 | |
| - | inside | Integron | - | - | 305566..368714 | 63148 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11234.97 Da Isoelectric Point: 9.7849
>T39872 WP_032477326.1 NZ_AP024968:322354-322641 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGMAFMEAWRNEQS
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGMAFMEAWRNEQS
Download Length: 288 bp
>T39872 NZ_AP024968:322354-322641 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGACGTTTCTATTTGCTAACC
ATTTACGGAATGGCTTTTATGGAGGCGTGGCGCAATGAGCAATCGTGA
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGACGTTTCTATTTGCTAACC
ATTTACGGAATGGCTTTTATGGAGGCGTGGCGCAATGAGCAATCGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 11696.20 Da Isoelectric Point: 6.7600
>AT39872 WP_000071008.1 NZ_AP024968:322628-322942 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL
Download Length: 315 bp
>AT39872 NZ_AP024968:322628-322942 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCACGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCACGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|