Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 313002..313617 Replicon chromosome
Accession NZ_AP024968
Organism Vibrio cholerae strain V130003

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag M2Z51_RS15310 Protein ID WP_032477340.1
Coordinates 313002..313289 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M2Z51_RS15315 Protein ID WP_032477342.1
Coordinates 313300..313617 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2Z51_RS15270 (V130003_29970) 308414..308728 + 315 WP_000248689.1 DUF4144 domain-containing protein -
M2Z51_RS15275 (V130003_29980) 308956..309237 + 282 WP_114732512.1 hypothetical protein -
M2Z51_RS15280 (V130003_29990) 309441..309830 + 390 WP_055052207.1 VOC family protein -
M2Z51_RS15285 (V130003_30000) 309970..310332 + 363 WP_248532149.1 SH3 domain-containing protein -
M2Z51_RS15290 310668..310820 + 153 WP_001893908.1 DUF645 family protein -
M2Z51_RS15295 (V130003_30010) 311213..311869 + 657 WP_000361704.1 quinolone resistance pentapeptide repeat protein QnrVC4 -
M2Z51_RS15300 (V130003_30020) 312036..312332 + 297 WP_069217115.1 Dabb family protein -
M2Z51_RS15305 (V130003_30030) 312488..312775 + 288 WP_032477325.1 hypothetical protein -
M2Z51_RS15310 (V130003_30040) 313002..313289 + 288 WP_032477340.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2Z51_RS15315 (V130003_30050) 313300..313617 + 318 WP_032477342.1 HigA family addiction module antitoxin Antitoxin
M2Z51_RS15320 (V130003_30060) 313818..314099 + 282 WP_032477343.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M2Z51_RS15325 (V130003_30070) 314096..314383 + 288 WP_000869999.1 type II toxin-antitoxin system RelE/ParE family toxin -
M2Z51_RS15330 (V130003_30080) 314538..315035 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
M2Z51_RS15335 (V130003_30090) 315032..315304 - 273 WP_000246253.1 DUF1778 domain-containing protein -
M2Z51_RS15340 315659..315841 + 183 WP_032477360.1 DUF645 family protein -
M2Z51_RS15345 (V130003_30100) 316496..316882 + 387 WP_029628680.1 MmcQ/YjbR family DNA-binding protein -
M2Z51_RS15350 (V130003_30110) 317024..317383 + 360 WP_001900224.1 hypothetical protein -
M2Z51_RS15355 (V130003_30120) 317533..317829 + 297 WP_001069046.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 304895..371259 66364
- inside Integron - - 305566..368714 63148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T39869 WP_032477340.1 NZ_AP024968:313002-313289 [Vibrio cholerae]
MTLEFKDKWLEQFYEDDKRHRLIPGSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T39869 NZ_AP024968:313002-313289 [Vibrio cholerae]
ATGACATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAGGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11732.70 Da        Isoelectric Point: 10.6985

>AT39869 WP_032477342.1 NZ_AP024968:313300-313617 [Vibrio cholerae]
MRKTKRRPVSVGQMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEVKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT39869 NZ_AP024968:313300-313617 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGCAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGTGTACACAGAAATACGGTGAGTAATTTGATTAATGGCGGAGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAATACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGTAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References