Detailed information of TA system TA039846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3369422..3370074
• Replicon: chromosome
• Accession: NZ_AP024948
• Organism: Aeromonas caviae strain KAM345

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDN68_RS15800
• Protein ID: WP_042046568.1
• Coordinates: 3369422..3369772 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDN68_RS15805
• Protein ID: WP_042046566.1
• Coordinates: 3369772..3370074 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN68_RS15780 (KAM345_030700)	3366114..3367154	-	1041	WP_042017433.1	IS481-like element ISAs19 family transposase	-
LDN68_RS15785	3367265..3368083	+	819	Protein_3046	IS3-like element ISAs17 family transposase	-
LDN68_RS15790	3368112..3368432	-	321	WP_158197741.1	hypothetical protein	-
LDN68_RS15795 (KAM345_030710)	3368424..3369374	+	951	WP_052815455.1	DUF932 domain-containing protein	-
LDN68_RS15800 (KAM345_030720)	3369422..3369772	+	351	WP_042046568.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDN68_RS15805 (KAM345_030730)	3369772..3370074	+	303	WP_042046566.1	XRE family transcriptional regulator	Antitoxin
LDN68_RS15810 (KAM345_030740)	3370251..3371243	+	993	WP_042046565.1	YqaJ viral recombinase family protein	-
LDN68_RS15815 (KAM345_030750)	3371249..3372187	+	939	WP_042046564.1	hypothetical protein	-
LDN68_RS15820	3372189..3372302	-	114	Protein_3053	transcriptional regulator	-
LDN68_RS15825 (KAM345_030760)	3372399..3372947	+	549	WP_052815456.1	lecithin retinol acyltransferase family protein	-
LDN68_RS15830 (KAM345_030770)	3372986..3373411	+	426	WP_042046560.1	DUF2787 domain-containing protein	-
LDN68_RS15835 (KAM345_030790)	3373825..3374091	+	267	WP_042046558.1	hypothetical protein	-
LDN68_RS15840 (KAM345_030800)	3374185..3374673	+	489	WP_042046556.1	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3355001..3378225	23224
	-	flank	IS/Tn	-	-	3374993..3376267	1274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13061.00 Da
• Isoelectric Point: 7.1672

>T39846 WP_042046568.1 NZ_AP024948:3369422-3369772 [Aeromonas caviae]
MWTVKTTELFNAWFDVQDEATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGEPLRAFFAFDPLRQAI
VLCAGNKGGNEKRFYKEMLPLAEAQYARHLAELEEK

Nucleotide

• Length: 351 bp

>T39846 NZ_AP024948:3369422-3369772 [Aeromonas caviae]
ATGTGGACAGTAAAAACGACCGAGCTGTTTAATGCTTGGTTTGATGTGCAAGATGAAGCGACGCAGGAGAAAGTCTTGGC
TGGGCTACTCGCCTTGGCACAAGGTGGCCCATCGGTAGGGCGTCCCCTGGTCGATACCATCAAGGGCTCGCGTTTTACAA
ACTTAAAAGAGCTGAGGGTGCAGCATCAGGGAGAGCCGCTACGTGCATTCTTTGCCTTTGACCCGCTGCGACAAGCCATC
GTGCTTTGTGCTGGCAACAAGGGCGGGAACGAGAAGCGGTTTTACAAAGAGATGCTGCCGTTGGCTGAGGCACAGTACGC
CAGGCATCTGGCTGAGTTGGAGGAAAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.90 Da
• Isoelectric Point: 5.2187

>AT39846 WP_042046566.1 NZ_AP024948:3369772-3370074 [Aeromonas caviae]
MGRTLDDLLASRSEESRQRIQEMATELLLEVRIQALREELEVSQVELAKALGVSQPAVVAMEQRGSDIKLSTMKRYVEAM
GGKLRVDVELPTGRHIGFNV

Nucleotide

• Length: 303 bp

>AT39846 NZ_AP024948:3369772-3370074 [Aeromonas caviae]
ATGGGAAGAACACTTGACGATTTGCTGGCCTCCCGTTCAGAAGAGAGTCGGCAACGCATCCAGGAAATGGCAACCGAGCT
GTTGCTGGAGGTGCGTATACAGGCGCTGCGCGAAGAGCTGGAGGTGTCGCAGGTTGAGTTGGCTAAGGCACTCGGTGTCA
GTCAGCCTGCCGTCGTGGCGATGGAGCAGCGTGGCAGTGATATCAAGCTCTCCACCATGAAACGCTATGTTGAAGCGATG
GGCGGCAAGCTGCGTGTAGATGTGGAATTGCCTACCGGCCGACACATCGGGTTCAACGTCTGA