Detailed information of TA system TA039770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 113496..114088
• Replicon: plasmid pCECT9189-1
• Accession: NZ_AP024856
• Organism: Photobacterium toruni strain CECT 9189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OC457_RS19805
• Protein ID: WP_080176478.1
• Coordinates: 113496..113816 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1T4UWE2
• Locus tag: OC457_RS19810
• Protein ID: WP_065176947.1
• Coordinates: 113813..114088 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OC457_RS19780	109799..110341	+	543	WP_080176482.1	hypothetical protein	-
OC457_RS19785	110486..111346	+	861	WP_080176481.1	nucleotide-binding protein	-
OC457_RS19790	111471..111767	+	297	WP_045037152.1	BrnT family toxin	-
OC457_RS19795	111760..111969	+	210	WP_080176480.1	BrnA antitoxin family protein	-
OC457_RS19800	112328..113095	-	768	WP_080176479.1	hypothetical protein	-
OC457_RS19805	113496..113816	+	321	WP_080176478.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OC457_RS19810	113813..114088	+	276	WP_065176947.1	helix-turn-helix transcriptional regulator	Antitoxin
OC457_RS19815	114894..115631	+	738	WP_262054089.1	IS6 family transposase	-
OC457_RS19820	115673..116050	+	378	WP_080176598.1	VOC family protein	-
OC457_RS19825	116404..116775	-	372	WP_262054090.1	transposase	-
OC457_RS19830	116854..117858	+	1005	WP_080176599.1	hypothetical protein	-
OC457_RS19835	117858..118355	+	498	WP_080176600.1	hypothetical protein	-
OC457_RS19840	118446..118976	+	531	WP_080176601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hcp1 / hcp1 / clpB/vasG / vasE / vasA / vipB/mglB / vipA/mglA / hcp1 / hcp1	1..217175	217175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12082.29 Da
• Isoelectric Point: 9.7089

>T39770 WP_080176478.1 NZ_AP024856:113496-113816 [Photobacterium toruni]
MAWKVDFYSGVEDEILAMPAKIQARMIKLLELIELYGANLGPPHTDSMGDGLFEIRAKAQEGIGRGLFCYLKGQHVYVLR
AFVKKTNKTPKKEIELARERMKEVKL

Nucleotide

• Length: 321 bp

>T39770 NZ_AP024856:113496-113816 [Photobacterium toruni]
ATGGCTTGGAAAGTGGATTTTTACTCTGGTGTTGAAGATGAAATATTAGCAATGCCAGCTAAAATTCAGGCTCGTATGAT
TAAGTTACTCGAATTAATTGAATTATATGGAGCAAATTTAGGCCCACCACATACAGACTCAATGGGAGATGGGCTTTTTG
AAATAAGAGCTAAAGCTCAAGAAGGTATAGGTAGAGGTTTGTTTTGCTATTTAAAAGGACAGCACGTTTACGTATTAAGA
GCTTTTGTGAAAAAGACGAACAAGACCCCAAAAAAAGAAATAGAACTTGCCAGAGAGCGAATGAAAGAGGTGAAGTTATG
A

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10004.48 Da
• Isoelectric Point: 8.9869

>AT39770 WP_065176947.1 NZ_AP024856:113813-114088 [Photobacterium toruni]
MSLSALKRKALQNPEVKEAYDDLSVEFELISSLISMREKSGLTQDEVAEKMGTKAPNISRLESGRSNPSLKTLVSYAQAC
GFKLNLGFKHA

Nucleotide

• Length: 276 bp

>AT39770 NZ_AP024856:113813-114088 [Photobacterium toruni]
ATGAGTTTATCTGCATTAAAAAGAAAAGCATTACAGAATCCTGAAGTAAAAGAAGCTTATGATGATCTAAGTGTTGAATT
TGAATTAATTAGTTCTCTAATTTCAATGAGAGAAAAATCAGGCTTAACTCAAGATGAAGTTGCTGAAAAAATGGGAACAA
AAGCTCCGAATATTTCACGCTTAGAATCTGGACGATCAAATCCTAGTCTTAAAACTTTAGTGAGTTATGCTCAAGCATGT
GGATTTAAGCTAAATCTTGGTTTTAAACATGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4UWE2