Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4828760..4829490 Replicon chromosome
Accession NZ_AP024847
Organism Serratia marcescens strain 2020-O-9

Toxin (Protein)


Gene name higB Uniprot ID A0A2S4X738
Locus tag LDN81_RS23365 Protein ID WP_033636137.1
Coordinates 4828760..4829071 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2V4GBG9
Locus tag LDN81_RS23370 Protein ID WP_033636138.1
Coordinates 4829068..4829490 (+) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN81_RS23335 (SMGES_46290) 4824071..4824796 + 726 WP_004937040.1 LPS export ABC transporter ATP-binding protein -
LDN81_RS23340 (SMGES_46300) 4824849..4826282 + 1434 WP_041036826.1 RNA polymerase factor sigma-54 -
LDN81_RS23345 (SMGES_46310) 4826306..4826593 + 288 WP_004937038.1 ribosome hibernation promoting factor -
LDN81_RS23350 (SMGES_46320) 4826749..4827210 + 462 WP_016930212.1 PTS IIA-like nitrogen regulatory protein PtsN -
LDN81_RS23355 (SMGES_46330) 4827340..4828194 + 855 WP_015379177.1 RNase adapter RapZ -
LDN81_RS23360 (SMGES_46340) 4828191..4828463 + 273 WP_033648996.1 PTS phosphocarrier protein NPr -
LDN81_RS23365 (SMGES_46350) 4828760..4829071 + 312 WP_033636137.1 type II toxin-antitoxin system HigB family toxin Toxin
LDN81_RS23370 (SMGES_46360) 4829068..4829490 + 423 WP_033636138.1 helix-turn-helix domain-containing protein Antitoxin
LDN81_RS23375 (SMGES_46370) 4829495..4831411 - 1917 WP_033648995.1 PRD domain-containing protein -
LDN81_RS23380 (SMGES_46380) 4831463..4832677 - 1215 WP_041036829.1 lactonase family protein -
LDN81_RS23385 (SMGES_46390) 4832772..4833512 - 741 WP_004937022.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(81-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12259.11 Da        Isoelectric Point: 10.0957

>T39764 WP_033636137.1 NZ_AP024847:4828760-4829071 [Serratia marcescens]
MHIISREPFNNATKQYPNEAAALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Download         Length: 312 bp

>T39764 NZ_AP024847:4828760-4829071 [Serratia marcescens]
ATGCATATCATTTCCAGGGAGCCGTTCAATAACGCAACCAAGCAATACCCTAATGAAGCCGCTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGCGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATTTTGTTCTTCGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAGTACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15676.97 Da        Isoelectric Point: 4.9590

>AT39764 WP_033636138.1 NZ_AP024847:4829068-4829490 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSSSQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAD

Download         Length: 423 bp

>AT39764 NZ_AP024847:4829068-4829490 [Serratia marcescens]
ATGATTACAGATGCCATTAAGGCCGCCGATGCGCTGCTGCGCGCCGTGCCCTTATTGCAGGGCAGCAGCTCTCAGCAAGA
CTACCGGGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AAATCGCCGAATATGAAGATAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAACTCCCGCAAGGAGTGGCC
GCGCTTCGCGTATTGATGGAACAACACCAACTTAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTCAG
CCAAATCCTGAGTGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCGGCCC
TCTTCCTGCCGGAAGCGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S4X738

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4GBG9

References