Detailed information of TA system TA039745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 543601..544254
• Replicon: chromosome
• Accession: NZ_AP024847
• Organism: Serratia marcescens strain 2020-O-9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDN81_RS02515
• Protein ID: WP_033645671.1
• Coordinates: 543601..543951 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: LDN81_RS02520
• Protein ID: WP_019453270.1
• Coordinates: 543955..544254 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN81_RS02475 (SMGES_04800)	538777..539187	-	411	WP_041037740.1	GNAT family N-acetyltransferase	-
LDN81_RS02480 (SMGES_04810)	539216..540586	-	1371	WP_041037742.1	glutamine synthetase family protein	-
LDN81_RS02485 (SMGES_04820)	540832..541149	+	318	WP_041037744.1	hypothetical protein	-
LDN81_RS02490 (SMGES_04830)	541140..541535	-	396	WP_041037746.1	hypothetical protein	-
LDN81_RS02495	541549..541941	-	393	WP_041037748.1	hypothetical protein	-
LDN81_RS02500 (SMGES_04840)	541969..542358	-	390	WP_033652822.1	hypothetical protein	-
LDN81_RS02505 (SMGES_04850)	542513..543199	-	687	WP_223915581.1	hypothetical protein	-
LDN81_RS02510 (SMGES_04860)	543168..543329	-	162	WP_223915583.1	hypothetical protein	-
LDN81_RS02515 (SMGES_04870)	543601..543951	+	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDN81_RS02520 (SMGES_04880)	543955..544254	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
LDN81_RS02525 (SMGES_04890)	544244..545161	-	918	WP_033648568.1	LysR family transcriptional regulator	-
LDN81_RS02530 (SMGES_04900)	545330..546028	+	699	WP_033648567.1	CoA transferase subunit A	-
LDN81_RS02535 (SMGES_04910)	546040..546693	+	654	WP_041037752.1	CoA transferase subunit B	-
LDN81_RS02540 (SMGES_04920)	546707..547891	+	1185	WP_041037754.1	acetyl-CoA C-acetyltransferase	-
LDN81_RS02545 (SMGES_04930)	547910..548833	+	924	WP_041037756.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T39745 WP_033645671.1 NZ_AP024847:543601-543951 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T39745 NZ_AP024847:543601-543951 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTCCGGCGGCCTTTTGTCGACGTGCTGAAAGGTTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATTGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT39745 WP_019453270.1 NZ_AP024847:543955-544254 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT39745 NZ_AP024847:543955-544254 [Serratia marcescens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAACGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3