Detailed information of TA system TA039739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 139037..139572
• Replicon: plasmid pAA622-1
• Accession: NZ_AP024838
• Organism: Enterococcus faecium strain AA622

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: LDN47_RS15670
• Protein ID: WP_002314366.1
• Coordinates: 139037..139309 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: LDN47_RS15675
• Protein ID: WP_002297422.1
• Coordinates: 139309..139572 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN47_RS15640 (GVanDAA622_32020)	135036..135653	-	618	WP_002376250.1	hypothetical protein	-
LDN47_RS15645 (GVanDAA622_32030)	135715..136395	-	681	Protein_148	IS6 family transposase	-
LDN47_RS15650 (GVanDAA622_32040)	136662..137210	-	549	WP_002292681.1	hypothetical protein	-
LDN47_RS15655 (GVanDAA622_32050)	137211..138068	-	858	WP_002292680.1	ParA family protein	-
LDN47_RS15660 (GVanDAA622_32060)	138354..138641	-	288	WP_002345819.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDN47_RS15665 (GVanDAA622_32070)	138631..138960	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
LDN47_RS15670 (GVanDAA622_32080)	139037..139309	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
LDN47_RS15675 (GVanDAA622_32090)	139309..139572	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDN47_RS15680 (GVanDAA622_32100)	139678..140604	-	927	WP_002328829.1	hypothetical protein	-
LDN47_RS15685	141428..141429	-	2	WP_002331383.1	IS6-like element IS1216 family transposase	-
LDN47_RS15690 (GVanDAA622_32130)	141845..142069	+	225	WP_002307648.1	DUF2933 domain-containing protein	-
LDN47_RS15695 (GVanDAA622_32140)	142072..142251	+	180	WP_002325573.1	DUF2933 domain-containing protein	-
LDN47_RS15700 (GVanDAA622_32150)	142365..143117	-	753	WP_002352399.1	transposase	-
LDN47_RS15705 (GVanDAA622_32160)	143202..143915	-	714	WP_002319971.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia	-	1..155411	155411
	-	inside	IScluster/Tn	-	-	122993..141744	18751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T39739 WP_002314366.1 NZ_AP024838:c139309-139037 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T39739 NZ_AP024838:c139309-139037 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT39739 WP_002297422.1 NZ_AP024838:c139572-139309 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT39739 NZ_AP024838:c139572-139309 [Enterococcus faecium]
ATGGCAAAAACTGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006