Detailed information of TA system TA039732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3185..3770
• Replicon: plasmid pOCUAc18-3
• Accession: NZ_AP024805
• Organism: Acinetobacter baumannii strain OCU Ac18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OCUAc18_RS19485
• Protein ID: WP_000897310.1
• Coordinates: 3450..3770 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCUAc18_RS19480
• Protein ID: WP_000369780.1
• Coordinates: 3185..3457 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAc18_RS19460 (OCUAc18_38140)	1..909	+	909	WP_026441244.1	replication initiation protein RepM	-
OCUAc18_RS19465 (OCUAc18_38150)	981..1502	+	522	WP_026441245.1	plasmid replication DNA-binding protein	-
OCUAc18_RS19470 (OCUAc18_38160)	1702..1938	-	237	WP_110975945.1	hypothetical protein	-
OCUAc18_RS19475 (OCUAc18_38170)	1965..2534	-	570	WP_016659739.1	hypothetical protein	-
OCUAc18_RS19480 (OCUAc18_38180)	3185..3457	-	273	WP_000369780.1	NadS family protein	Antitoxin
OCUAc18_RS19485 (OCUAc18_38190)	3450..3770	-	321	WP_000897310.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAc18_RS19490 (OCUAc18_38200)	3957..4154	-	198	WP_000476222.1	hypothetical protein	-
OCUAc18_RS19495 (OCUAc18_38210)	4221..4448	-	228	WP_000921865.1	hypothetical protein	-
OCUAc18_RS19500 (OCUAc18_38220)	4546..4761	-	216	WP_000071892.1	cold-shock protein	-
OCUAc18_RS19505 (OCUAc18_38230)	5117..5512	-	396	WP_004759377.1	type II toxin-antitoxin system VapC family toxin	-
OCUAc18_RS19510 (OCUAc18_38240)	5509..5739	-	231	WP_004282079.1	type II toxin-antitoxin system VapB family antitoxin	-
OCUAc18_RS19515 (OCUAc18_38250)	6370..7653	+	1284	WP_151838536.1	hypothetical protein	-
OCUAc18_RS19520 (OCUAc18_38260)	7703..7924	-	222	WP_087548429.1	hypothetical protein	-
OCUAc18_RS19525 (OCUAc18_38270)	7921..8277	-	357	WP_221255522.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10831	10831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (40-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12208.02 Da
• Isoelectric Point: 8.8648

>T39732 WP_000897310.1 NZ_AP024805:c3770-3450 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGNLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T39732 NZ_AP024805:c3770-3450 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCAACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT39732 WP_000369780.1 NZ_AP024805:c3457-3185 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT39732 NZ_AP024805:c3457-3185 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA