Detailed information of TA system TA039726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2727..3378
• Replicon: plasmid pOCUAc17-3
• Accession: NZ_AP024801
• Organism: Acinetobacter pittii strain OCU Ac17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A062G190
• Locus tag: OCUAc17_RS19610
• Protein ID: WP_005065055.1
• Coordinates: 2727..3071 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCUAc17_RS19615
• Protein ID: WP_005065057.1
• Coordinates: 3076..3378 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAc17_RS19595 (OCUAc17_38230)	1..930	+	930	WP_005065049.1	replication initiation protein RepM	-
OCUAc17_RS19600 (OCUAc17_38240)	1009..1539	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
OCUAc17_RS19605 (OCUAc17_38250)	1889..2206	+	318	WP_000900860.1	hypothetical protein	-
OCUAc17_RS19610 (OCUAc17_38260)	2727..3071	+	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAc17_RS19615 (OCUAc17_38270)	3076..3378	+	303	WP_005065057.1	XRE family transcriptional regulator	Antitoxin
OCUAc17_RS19620 (OCUAc17_38280)	3470..3871	+	402	WP_005065059.1	hypothetical protein	-
OCUAc17_RS19625 (OCUAc17_38290)	3923..4597	+	675	WP_005065062.1	HEPN domain-containing protein	-
OCUAc17_RS19630 (OCUAc17_38300)	4688..4999	-	312	WP_005065064.1	BrnA antitoxin family protein	-
OCUAc17_RS19635 (OCUAc17_38310)	4986..5273	-	288	WP_002124950.1	BrnT family toxin	-
OCUAc17_RS19640 (OCUAc17_38320)	5786..7273	+	1488	WP_005065066.1	SulP family inorganic anion transporter	-
OCUAc17_RS19645 (OCUAc17_38330)	7286..8137	+	852	WP_005065068.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11158	11158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13143.23 Da
• Isoelectric Point: 6.4651

>T39726 WP_005065055.1 NZ_AP024801:2727-3071 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADKQYELHLSTLGE

Nucleotide

• Length: 345 bp

>T39726 NZ_AP024801:2727-3071 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAATAACACAAGAGAAAGTCTTAGC
TGCGTTGGTTGTTCTGCAACTGCAAGGACCAAGTTTAGGTCGTCCCTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGAGGTAAACCATTAAGAGCATTTTTTGCATTCGATCCATTACGACAAGCAATT
GTACTGTGTATTGGTGATAAGAGCGGTAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATAAGCAATATGAACT
TCATCTCTCAACATTAGGAGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 5.8961

>AT39726 WP_005065057.1 NZ_AP024801:3076-3378 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT39726 NZ_AP024801:3076-3378 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCTGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
GCTATTAGAAAATCAGCTTTACCTTATTCGTGAAGAACTCGAAATCTCTCAAAAAGAACTGGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAAGCTATG
GGAGGAAAGCTCCGTATTGATGTGGAACTTCCAACTGGGAAACATATAGGATTCAATGTATAG

Structures

Toxin

• AlphaFold DB: A0A062G190