Detailed information of TA system TA039723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 18906..19347
• Replicon: plasmid pOCUAc17-1
• Accession: NZ_AP024799
• Organism: Acinetobacter pittii strain OCU Ac17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OCUAc17_RS19210
• Protein ID: WP_002072926.1
• Coordinates: 19180..19347 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCUAc17_RS19205
• Protein ID: WP_001258992.1
• Coordinates: 18906..19193 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAc17_RS19170 (OCUAc17_37390)	14283..14738	+	456	WP_005071565.1	hypothetical protein	-
OCUAc17_RS19175 (OCUAc17_37400)	14799..15266	+	468	WP_005071563.1	hypothetical protein	-
OCUAc17_RS19180 (OCUAc17_37410)	15235..15876	+	642	WP_005071561.1	putative metallopeptidase	-
OCUAc17_RS19185	15936..16477	+	542	Protein_16	DUF2280 domain-containing protein	-
OCUAc17_RS19190 (OCUAc17_37440)	16461..17777	+	1317	WP_086376539.1	terminase	-
OCUAc17_RS19195 (OCUAc17_37450)	17817..18374	+	558	Protein_18	hypothetical protein	-
OCUAc17_RS19200 (OCUAc17_37460)	18398..18622	+	225	WP_016653391.1	hypothetical protein	-
OCUAc17_RS19205 (OCUAc17_37480)	18906..19193	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
OCUAc17_RS19210 (OCUAc17_37490)	19180..19347	-	168	WP_002072926.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAc17_RS19215 (OCUAc17_37500)	19645..19965	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
OCUAc17_RS19220	20333..20539	+	207	WP_242439879.1	AAA family ATPase	-
OCUAc17_RS19225	20467..21012	+	546	Protein_24	IS5 family transposase	-
OCUAc17_RS19230 (OCUAc17_37530)	21770..21997	+	228	WP_005071544.1	hypothetical protein	-
OCUAc17_RS19235 (OCUAc17_37540)	22107..22316	-	210	WP_000069471.1	hypothetical protein	-
OCUAc17_RS19240 (OCUAc17_37550)	22309..22611	-	303	WP_001140619.1	XRE family transcriptional regulator	-
OCUAc17_RS19245 (OCUAc17_37560)	22604..22960	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OCUAc17_RS19250 (OCUAc17_37570)	23670..24206	+	537	WP_009501148.1	porin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..69156	69156
	-	flank	IS/Tn	-	-	20767..21012	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-48)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6362.64 Da
• Isoelectric Point: 11.0180

>T39723 WP_002072926.1 NZ_AP024799:c19347-19180 [Acinetobacter pittii]
MRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 168 bp

>T39723 NZ_AP024799:c19347-19180 [Acinetobacter pittii]
TTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAAACGCATTGTAATGCTCCATAG
TTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCATGAAAGAGGTGAAAAATGCGA
ACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT39723 WP_001258992.1 NZ_AP024799:c19193-18906 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT39723 NZ_AP024799:c19193-18906 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCTTGCTCGATATGGTACTTACTGCACTTCGAGAGGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAATGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATACGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA