Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 351689..352335 Replicon chromosome
Accession NZ_AP024784
Organism Klebsiella pneumoniae strain TUM12137

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag K6K11_RS01620 Protein ID WP_002920560.1
Coordinates 351689..352036 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K6K11_RS01625 Protein ID WP_002920557.1
Coordinates 352036..352335 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6K11_RS01610 (TUM12137_03150) 347615..349048 + 1434 WP_002920564.1 glycogen synthase GlgA -
K6K11_RS01615 (TUM12137_03160) 349066..351513 + 2448 WP_002920561.1 glycogen phosphorylase -
K6K11_RS01620 (TUM12137_03170) 351689..352036 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6K11_RS01625 (TUM12137_03180) 352036..352335 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K6K11_RS01630 (TUM12137_03190) 352398..353906 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K6K11_RS01635 (TUM12137_03200) 354111..354440 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K6K11_RS01640 (TUM12137_03210) 354491..355321 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K6K11_RS01645 (TUM12137_03220) 355371..356129 + 759 WP_221273938.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T39637 WP_002920560.1 NZ_AP024784:351689-352036 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T39637 NZ_AP024784:351689-352036 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT39637 WP_002920557.1 NZ_AP024784:352036-352335 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT39637 NZ_AP024784:352036-352335 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References