Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2578758..2579404 Replicon chromosome
Accession NZ_AP024756
Organism Klebsiella pneumoniae strain TUM12128

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag K6J96_RS12865 Protein ID WP_002920560.1
Coordinates 2579057..2579404 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K6J96_RS12860 Protein ID WP_002920557.1
Coordinates 2578758..2579057 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J96_RS12840 (TUM12128_24570) 2574964..2575722 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
K6J96_RS12845 (TUM12128_24580) 2575772..2576602 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K6J96_RS12850 (TUM12128_24590) 2576653..2576982 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K6J96_RS12855 (TUM12128_24600) 2577187..2578695 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K6J96_RS12860 (TUM12128_24610) 2578758..2579057 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K6J96_RS12865 (TUM12128_24620) 2579057..2579404 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6J96_RS12870 (TUM12128_24630) 2579580..2582027 - 2448 WP_002920561.1 glycogen phosphorylase -
K6J96_RS12875 (TUM12128_24640) 2582045..2583478 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T39476 WP_002920560.1 NZ_AP024756:c2579404-2579057 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T39476 NZ_AP024756:c2579404-2579057 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT39476 WP_002920557.1 NZ_AP024756:c2579057-2578758 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT39476 NZ_AP024756:c2579057-2578758 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References