Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 2328658..2329299 Replicon chromosome
Accession NZ_AP024718
Organism Methylomarinovum sp. IN45

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag MIN45_RS11735 Protein ID WP_286292461.1
Coordinates 2329018..2329299 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MIN45_RS11730 Protein ID WP_286292459.1
Coordinates 2328658..2329008 (-) Length 117 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MIN45_RS11710 (MIN45_P2254) 2324854..2325933 + 1080 WP_286292448.1 acyl-CoA dehydrogenase family protein -
MIN45_RS11715 (MIN45_P2255) 2325985..2327295 - 1311 WP_286292455.1 tryptophan 7-halogenase -
MIN45_RS11720 (MIN45_P2256) 2327417..2328349 - 933 WP_286292457.1 restriction endonuclease -
MIN45_RS11725 2328348..2328485 + 138 Protein_2291 IS256 family transposase -
MIN45_RS11730 (MIN45_P2257) 2328658..2329008 - 351 WP_286292459.1 HigA family addiction module antitoxin Antitoxin
MIN45_RS11735 2329018..2329299 - 282 WP_286292461.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MIN45_RS11740 (MIN45_P2259) 2329409..2330584 - 1176 WP_286292462.1 NAD(P)/FAD-dependent oxidoreductase -
MIN45_RS11745 (MIN45_P2260) 2330581..2332491 - 1911 WP_286292463.1 beta-ketoacyl-ACP synthase -
MIN45_RS11750 (MIN45_P2261) 2332488..2332868 - 381 WP_286292465.1 rhodanese-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-85)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10874.39 Da        Isoelectric Point: 10.2176

>T39248 WP_286292461.1 NZ_AP024718:c2329299-2329018 [Methylomarinovum sp. IN45]
VIRSFRDKRTAAIFQGLFVSGLPKSMQPRALDKLRMIHNAATIEDLRSPPGNRLEKLTGNRSGQYSIRINQQWRICFRWE
EGDAWDVEITDYH

Download         Length: 282 bp

>T39248 NZ_AP024718:c2329299-2329018 [Methylomarinovum sp. IN45]
GTGATCCGAAGCTTCAGAGACAAACGAACAGCAGCAATCTTTCAGGGGTTGTTCGTCAGCGGTCTTCCGAAATCCATGCA
GCCTAGAGCTTTGGATAAACTCAGGATGATTCACAATGCCGCCACGATCGAGGATCTGCGCTCACCCCCGGGCAACCGTC
TGGAAAAGCTCACAGGCAATCGGTCGGGGCAATACAGCATCCGGATCAACCAGCAATGGCGCATCTGTTTCCGCTGGGAG
GAAGGAGATGCCTGGGATGTGGAAATCACCGATTATCATTGA

Antitoxin


Download         Length: 117 a.a.        Molecular weight: 13020.99 Da        Isoelectric Point: 7.0171

>AT39248 WP_286292459.1 NZ_AP024718:c2329008-2328658 [Methylomarinovum sp. IN45]
MSISFEELSRTDYSDITTGEVIPISTPGEILRREFMEPLGLSANALAKALKVPANRITAILHGGRSITADTALRLGRYFG
TTAEFWINLQKDYELRLARRKLEKQIEIEVKPLESA

Download         Length: 351 bp

>AT39248 NZ_AP024718:c2329008-2328658 [Methylomarinovum sp. IN45]
ATGAGCATTTCGTTTGAAGAACTGTCCCGAACCGATTATTCCGACATCACGACCGGAGAAGTCATCCCCATCAGCACGCC
TGGCGAGATTCTGCGCCGGGAGTTCATGGAACCCTTGGGGTTGTCGGCCAACGCCCTTGCCAAAGCCCTGAAGGTGCCTG
CCAACCGGATCACCGCCATCCTCCATGGAGGCCGTTCCATCACTGCCGATACGGCGCTGCGGCTTGGGCGCTATTTCGGC
ACCACGGCGGAATTCTGGATCAACCTGCAGAAGGATTACGAACTGCGGCTGGCACGCCGCAAATTGGAAAAACAGATTGA
AATAGAGGTTAAACCGCTGGAGTCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References