Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2093729..2094319 Replicon chromosome
Accession NZ_AP024718
Organism Methylomarinovum sp. IN45

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MIN45_RS10540 Protein ID WP_286292060.1
Coordinates 2093729..2094007 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MIN45_RS10545 Protein ID WP_286292061.1
Coordinates 2094020..2094319 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MIN45_RS10520 (MIN45_P2021) 2089277..2091292 - 2016 WP_286292056.1 TonB-dependent receptor -
MIN45_RS10525 (MIN45_P2022) 2091366..2091701 - 336 WP_286292057.1 hydrogenase maturation nickel metallochaperone HypA -
MIN45_RS10530 (MIN45_P2023) 2091679..2092128 - 450 WP_286292058.1 hydrogenase maturation protease -
MIN45_RS10535 (MIN45_P2024) 2092125..2093630 - 1506 WP_286292059.1 Ni/Fe hydrogenase subunit alpha -
MIN45_RS10540 (MIN45_P2025) 2093729..2094007 + 279 WP_286292060.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MIN45_RS10545 (MIN45_P2026) 2094020..2094319 + 300 WP_286292061.1 HigA family addiction module antitoxin Antitoxin
MIN45_RS10550 (MIN45_P2027) 2094336..2094875 - 540 WP_286292063.1 NADP oxidoreductase -
MIN45_RS10555 (MIN45_P2028) 2094872..2095195 - 324 WP_286292064.1 nucleotidyltransferase domain-containing protein -
MIN45_RS10560 (MIN45_P2029) 2095192..2095587 - 396 WP_286292065.1 nucleotidyltransferase substrate binding protein -
MIN45_RS10565 (MIN45_P2030) 2095580..2096302 - 723 WP_286292066.1 2Fe-2S iron-sulfur cluster-binding protein -
MIN45_RS10570 (MIN45_P2031) 2096307..2098079 - 1773 WP_286292067.1 NAD(P)H-dependent oxidoreductase subunit E -
MIN45_RS10575 (MIN45_P2032) 2098090..2099094 - 1005 WP_286292068.1 hydrogenase expression/formation protein HypE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-66)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10902.36 Da        Isoelectric Point: 9.2970

>T39247 WP_286292060.1 NZ_AP024718:2093729-2094007 [Methylomarinovum sp. IN45]
MITSFRCKETRALFEGGDPKRFRSIRKVAERKLTQLHNAATLDFLRAPPGNRLEKLSGDREGQWSIRINDQWRICFTWHE
GEVRDVEITDYH

Download         Length: 279 bp

>T39247 NZ_AP024718:2093729-2094007 [Methylomarinovum sp. IN45]
ATGATCACCTCGTTTCGATGCAAGGAAACCCGGGCGCTTTTCGAAGGCGGCGATCCCAAGCGCTTCCGTAGCATCCGGAA
AGTCGCGGAACGCAAACTGACACAATTGCACAACGCCGCCACATTGGACTTCCTACGGGCACCACCGGGTAACCGCCTGG
AGAAACTCAGCGGCGACCGGGAAGGTCAATGGAGTATCCGCATCAACGACCAGTGGCGTATCTGCTTCACCTGGCACGAG
GGTGAAGTGCGGGACGTGGAAATCACCGATTATCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11241.99 Da        Isoelectric Point: 10.3338

>AT39247 WP_286292061.1 NZ_AP024718:2094020-2094319 [Methylomarinovum sp. IN45]
MIKNGMRPVHPGEVLREDYLKPLGLSVNALARALRVPPTRLHEIVNERRGITPDTALRLARYFGGDARSWLNLQAEYDLK
IVEREKARQIEAEVSPRAA

Download         Length: 300 bp

>AT39247 NZ_AP024718:2094020-2094319 [Methylomarinovum sp. IN45]
ATGATCAAGAACGGCATGCGACCGGTTCATCCCGGCGAGGTTCTGCGGGAGGATTACCTGAAGCCCTTGGGGCTCAGCGT
CAACGCTTTGGCCCGGGCGTTGCGCGTGCCACCGACACGGCTTCATGAAATCGTCAACGAACGTCGTGGCATCACGCCCG
ATACCGCCCTGCGTCTGGCCCGCTATTTCGGTGGCGACGCCCGCTCCTGGCTCAATCTGCAGGCGGAATACGACCTGAAA
ATCGTGGAACGGGAAAAAGCCCGCCAGATCGAGGCAGAGGTTTCGCCCCGGGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References