Detailed information of TA system TA039241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6126..6777
• Replicon: plasmid pTol5
• Accession: NZ_AP024709
• Organism: Acinetobacter sp. Tol 5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: TOL5_RS21755
• Protein ID: WP_004668307.1
• Coordinates: 6126..6482 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TOL5_RS21760
• Protein ID: WP_005237493.1
• Coordinates: 6475..6777 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL5_RS21740 (TOL5_42870)	1632..2795	+	1164	WP_004726779.1	ArsO family NAD(P)H-dependent flavin-containing monooxygenase	-
TOL5_RS21745 (TOL5_42880)	2958..3809	-	852	WP_004726781.1	universal stress protein	-
TOL5_RS21750 (TOL5_42890)	3822..5309	-	1488	WP_004726783.1	SulP family inorganic anion transporter	-
TOL5_RS21755 (TOL5_42900)	6126..6482	+	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOL5_RS21760 (TOL5_42910)	6475..6777	+	303	WP_005237493.1	XRE family transcriptional regulator	Antitoxin
TOL5_RS21765 (TOL5_42920)	6819..6980	-	162	Protein_7	IS6 family transposase	-
TOL5_RS21770 (TOL5_42930)	7074..7466	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
TOL5_RS21775 (TOL5_42940)	7556..8836	+	1281	WP_005237495.1	cation transporter	-
TOL5_RS21780 (TOL5_42950)	8922..9116	+	195	Protein_10	IS6 family transposase	-
TOL5_RS21785 (TOL5_42960)	9231..9695	-	465	Protein_11	IS5-like element ISAha2 family transposase	-
TOL5_RS21790 (TOL5_42970)	9940..10995	-	1056	WP_000991568.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117717	117717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T39241 WP_004668307.1 NZ_AP024709:6126-6482 [Acinetobacter sp. Tol 5]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T39241 NZ_AP024709:6126-6482 [Acinetobacter sp. Tol 5]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11208.99 Da
• Isoelectric Point: 6.4759

>AT39241 WP_005237493.1 NZ_AP024709:6475-6777 [Acinetobacter sp. Tol 5]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTLKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT39241 NZ_AP024709:6475-6777 [Acinetobacter sp. Tol 5]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCTTGAAAAAGTATGTTGAGGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: N9NM16