Detailed information of TA system TA039232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4912719..4913321
• Replicon: chromosome
• Accession: NZ_AP024687
• Organism: Escherichia coli strain FUJ80154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FJMB_RS23610
• Protein ID: WP_000897305.1
• Coordinates: 4913010..4913321 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FJMB_RS23605
• Protein ID: WP_000356397.1
• Coordinates: 4912719..4913009 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJMB_RS23585 (4909221)	4909221..4910123	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FJMB_RS23590 (4910120)	4910120..4910755	+	636	WP_039052566.1	formate dehydrogenase cytochrome b556 subunit	-
FJMB_RS23595 (4910752)	4910752..4911681	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FJMB_RS23600 (4911896)	4911896..4912114	-	219	WP_001298592.1	CopG family transcriptional regulator	-
FJMB_RS23605 (4912719)	4912719..4913009	-	291	WP_000356397.1	NadS family protein	Antitoxin
FJMB_RS23610 (4913010)	4913010..4913321	-	312	WP_000897305.1	hypothetical protein	Toxin
FJMB_RS23615 (4913550)	4913550..4914458	+	909	WP_001318161.1	alpha/beta hydrolase	-
FJMB_RS23620 (4914522)	4914522..4915463	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FJMB_RS23625 (4915508)	4915508..4915945	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
FJMB_RS23630 (4915942)	4915942..4916814	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FJMB_RS23635 (4916808)	4916808..4917407	-	600	WP_001314324.1	glucose-1-phosphatase	-
FJMB_RS23640 (4917506)	4917506..4918291	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T39232 WP_000897305.1 NZ_AP024687:c4913321-4913010 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T39232 NZ_AP024687:c4913321-4913010 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT39232 WP_000356397.1 NZ_AP024687:c4913009-4912719 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT39232 NZ_AP024687:c4913009-4912719 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7