Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3982935..3983629 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag FJMB_RS19215 Protein ID WP_001263491.1
Coordinates 3982935..3983333 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag FJMB_RS19220 Protein ID WP_000554755.1
Coordinates 3983336..3983629 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS19185 (3978197) 3978197..3979654 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
FJMB_RS19190 (3979663) 3979663..3979944 + 282 WP_022645226.1 hypothetical protein -
FJMB_RS19195 (3979961) 3979961..3980470 - 510 WP_001361775.1 metal-dependent hydrolase -
FJMB_RS19200 (3980532) 3980532..3981146 - 615 WP_022645225.1 peptide chain release factor H -
FJMB_RS19205 (3981143) 3981143..3982282 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
FJMB_RS19210 (3982473) 3982473..3982925 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
FJMB_RS19215 (3982935) 3982935..3983333 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FJMB_RS19220 (3983336) 3983336..3983629 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FJMB_RS19225 (3983681) 3983681..3984736 - 1056 WP_022645222.1 DNA polymerase IV -
FJMB_RS19230 (3984807) 3984807..3985592 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
FJMB_RS19235 (3985564) 3985564..3987276 + 1713 Protein_3768 flagellar biosynthesis protein FlhA -
FJMB_RS19240 (3987374) 3987374..3988147 - 774 WP_022645219.1 C40 family peptidase -
FJMB_RS19245 (3988333) 3988333..3988593 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T39223 WP_001263491.1 NZ_AP024687:c3983333-3982935 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T39223 NZ_AP024687:c3983333-3982935 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT39223 WP_000554755.1 NZ_AP024687:c3983629-3983336 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT39223 NZ_AP024687:c3983629-3983336 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References