Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2177024..2177892 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag K5645_RS10025 Protein ID WP_010886136.1
Coordinates 2177024..2177401 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag K5645_RS10030 Protein ID WP_003409886.1
Coordinates 2177443..2177892 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS09975 2172813..2173265 - 453 WP_003899095.1 lipoprotein -
K5645_RS09980 (MTR2092_19930) 2173329..2173730 + 402 WP_003409869.1 hypothetical protein -
K5645_RS09985 2173723..2173905 - 183 WP_003409870.1 hypothetical protein -
K5645_RS09990 (MTR2092_19940) 2174019..2174369 - 351 WP_003409871.1 hypothetical protein -
K5645_RS09995 2174380..2175282 - 903 WP_003899097.1 hypothetical protein -
K5645_RS10000 (MTR2092_19970) 2175303..2175494 - 192 WP_003409876.1 hypothetical protein -
K5645_RS10005 (MTR2092_19980) 2175495..2175791 - 297 WP_003409877.1 hypothetical protein -
K5645_RS10010 (MTR2092_19990) 2176031..2176246 + 216 WP_003409878.1 antitoxin -
K5645_RS10015 (MTR2092_20000) 2176243..2176554 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
K5645_RS10020 2176528..2177049 - 522 WP_003904745.1 hypothetical protein -
K5645_RS10025 (MTR2092_20020) 2177024..2177401 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
K5645_RS10030 (MTR2092_20030) 2177443..2177892 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K5645_RS10035 (MTR2092_20040) 2177889..2178434 + 546 WP_003409891.1 SecB-like chaperone -
K5645_RS10040 2178323..2178937 - 615 WP_003901296.1 hypothetical protein -
K5645_RS10045 (MTR2092_20060) 2178986..2179282 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
K5645_RS10050 (MTR2092_20070) 2179279..2179530 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
K5645_RS10055 (MTR2092_20080) 2179517..2180011 + 495 WP_003899099.1 hypothetical protein -
K5645_RS10060 (MTR2092_20090) 2180171..2180578 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
K5645_RS10065 (MTR2092_20100) 2180582..2180854 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K5645_RS10070 (MTR2092_20110) 2180887..2182107 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T39175 WP_010886136.1 NZ_AP024671:2177024-2177401 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T39175 NZ_AP024671:2177024-2177401 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT39175 WP_003409886.1 NZ_AP024671:2177443-2177892 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT39175 NZ_AP024671:2177443-2177892 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References