Detailed information of TA system TA038876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4196080..4196896
• Replicon: chromosome
• Accession: NZ_AP024585
• Organism: Citrobacter amalonaticus strain JCM 1661

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: KI228_RS19890
• Protein ID: WP_061069544.1
• Coordinates: 4196080..4196556 (-)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A3Q8DNJ3
• Locus tag: KI228_RS19895
• Protein ID: WP_042999108.1
• Coordinates: 4196561..4196896 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI228_RS19870 (CIAM_38930)	4191185..4191928	-	744	WP_044264050.1	molecular chaperone	-
KI228_RS19875 (CIAM_38940)	4191971..4194415	-	2445	WP_224267598.1	fimbria/pilus outer membrane usher protein	-
KI228_RS19880 (CIAM_38950)	4194509..4195057	-	549	WP_042999111.1	fimbrial protein	-
KI228_RS19885 (CIAM_38960)	4195126..4195656	-	531	WP_042999110.1	fimbrial protein	-
KI228_RS19890 (CIAM_38970)	4196080..4196556	-	477	WP_061069544.1	type II toxin-antitoxin system YhaV family toxin	Toxin
KI228_RS19895 (CIAM_38980)	4196561..4196896	-	336	WP_042999108.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
KI228_RS19900 (CIAM_38990)	4197111..4197647	-	537	WP_042999107.1	ferredoxin-type protein NapF	-
KI228_RS19905 (CIAM_39000)	4197644..4198240	-	597	WP_042999106.1	molecular chaperone	-
KI228_RS19910 (CIAM_39010)	4198285..4199058	-	774	WP_044257303.1	dimethyl sulfoxide reductase anchor subunit	-
KI228_RS19915 (CIAM_39020)	4199051..4199677	-	627	WP_042999104.1	dimethylsulfoxide reductase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (15-110)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18194.81 Da
• Isoelectric Point: 8.4404

>T38876 WP_061069544.1 NZ_AP024585:c4196556-4196080 [Citrobacter amalonaticus]
MDFPTWLNGWTIYAHPCFIDQYNALVARVEDLKHRFPDPIVFQKKKETKVLAHLLKSIANITHEPRASVYLPGDSIGKAY
TDWSRAKFGGGRYRLFFRYSLESKIIVIAWVNDEGPLRTYGSKTDAYKIFGKMLDEGNPPDDWLSLLQACQNDGKEHL

Nucleotide

• Length: 477 bp

>T38876 NZ_AP024585:c4196556-4196080 [Citrobacter amalonaticus]
ATGGATTTTCCCACATGGCTGAACGGGTGGACGATCTACGCTCATCCCTGTTTTATCGATCAATACAACGCGCTGGTCGC
ACGTGTTGAAGACCTTAAGCACAGGTTTCCAGATCCCATTGTTTTCCAGAAGAAGAAAGAGACCAAGGTGCTGGCGCATT
TATTGAAATCCATTGCCAACATTACCCACGAACCCCGAGCTTCTGTTTACCTGCCTGGTGACAGTATAGGGAAAGCTTAT
ACAGACTGGTCGAGAGCGAAGTTTGGTGGGGGACGGTATCGCTTATTTTTCCGTTATAGTCTCGAGAGTAAGATCATTGT
CATCGCATGGGTGAATGATGAAGGGCCATTGCGCACCTACGGCAGCAAGACCGATGCCTATAAGATTTTCGGGAAAATGC
TCGATGAGGGGAATCCACCCGACGACTGGCTAAGTTTGCTTCAGGCTTGTCAAAACGACGGGAAGGAACATCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.83 Da
• Isoelectric Point: 4.1876

>AT38876 WP_042999108.1 NZ_AP024585:c4196896-4196561 [Citrobacter amalonaticus]
MFAYVRSEDIAIEADSKLTERHQTTIPASIRDALHLRSGDRIHYTLLSTGEVLLSKYSEATEDGVMRSFLDFLSQDVANN
PQTLKKLDLSRGYELVDGIDVNLDDEISDED

Nucleotide

• Length: 336 bp

>AT38876 NZ_AP024585:c4196896-4196561 [Citrobacter amalonaticus]
ATGTTCGCCTATGTTCGATCTGAAGACATTGCCATTGAAGCGGATTCTAAATTGACGGAACGACATCAAACGACTATCCC
TGCGTCGATCAGGGACGCTTTACATCTTCGCAGTGGTGATCGTATTCATTACACATTGCTTTCCACGGGTGAAGTACTGC
TTTCAAAATACAGTGAAGCAACGGAGGACGGTGTGATGCGATCATTCCTGGACTTCTTATCACAGGATGTGGCGAATAAC
CCACAAACGCTGAAGAAGCTGGATTTATCCCGTGGCTATGAACTGGTTGATGGTATCGACGTCAATCTTGATGATGAAAT
CAGCGACGAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DNJ3