Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 51318..52045 Replicon plasmid P1
Accession NZ_AP024580
Organism Klebsiella pneumoniae strain KpWEA4-2

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag L6443_RS26295 Protein ID WP_004118600.1
Coordinates 51318..51629 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag L6443_RS26300 Protein ID WP_000990392.1
Coordinates 51626..52045 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6443_RS26275 (MAKP6SUB2_50570) 47832..48551 + 720 WP_001102107.1 (Fe-S)-binding protein -
L6443_RS26280 (MAKP6SUB2_50580) 48562..49989 + 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
L6443_RS26285 (MAKP6SUB2_50590) 49982..50677 + 696 WP_004118595.1 lactate utilization protein C -
L6443_RS26290 (MAKP6SUB2_50600) 50727..51107 - 381 Protein_46 gluconate permease -
L6443_RS26295 (MAKP6SUB2_50610) 51318..51629 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
L6443_RS26300 (MAKP6SUB2_50620) 51626..52045 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
L6443_RS26305 (MAKP6SUB2_50630) 52082..53284 - 1203 WP_003031541.1 hypothetical protein -
L6443_RS26310 (MAKP6SUB2_50640) 53277..53606 - 330 WP_004118613.1 hypothetical protein -
L6443_RS26315 (MAKP6SUB2_50650) 53603..54262 - 660 WP_000078540.1 hypothetical protein -
L6443_RS26320 54686..55042 - 357 WP_077253206.1 Ref family recombination enhancement nuclease -
L6443_RS26325 55162..55302 - 141 WP_004118614.1 hypothetical protein -
L6443_RS26330 (MAKP6SUB2_50670) 55595..55948 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
L6443_RS26335 (MAKP6SUB2_50680) 55996..56358 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 / tet(A) / dfrA1 / qacE / sul1 / aac(3)-IId / blaLAP-2 / qnrS1 - 1..220075 220075


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T38860 WP_004118600.1 NZ_AP024580:51318-51629 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T38860 NZ_AP024580:51318-51629 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT38860 WP_000990392.1 NZ_AP024580:51626-52045 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT38860 NZ_AP024580:51626-52045 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References