Detailed information of TA system TA038768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 358669..359315
• Replicon: chromosome
• Accession: NZ_AP024568
• Organism: Klebsiella pneumoniae strain KpWEA1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L6449_RS01665
• Protein ID: WP_032415766.1
• Coordinates: 358669..359016 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: L6449_RS01670
• Protein ID: WP_002920557.1
• Coordinates: 359016..359315 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6449_RS01655 (MAKP3_03230)	354595..356028	+	1434	WP_002920564.1	glycogen synthase GlgA	-
L6449_RS01660 (MAKP3_03240)	356046..358493	+	2448	WP_002920561.1	glycogen phosphorylase	-
L6449_RS01665 (MAKP3_03250)	358669..359016	+	348	WP_032415766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6449_RS01670 (MAKP3_03260)	359016..359315	+	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
L6449_RS01675 (MAKP3_03270)	359378..360886	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
L6449_RS01680 (MAKP3_03280)	361091..361420	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
L6449_RS01685 (MAKP3_03290)	361471..362301	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
L6449_RS01690 (MAKP3_03300)	362351..363109	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13535.49 Da
• Isoelectric Point: 5.2054

>T38768 WP_032415766.1 NZ_AP024568:358669-359016 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNENRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T38768 NZ_AP024568:358669-359016 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAATCGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT38768 WP_002920557.1 NZ_AP024568:359016-359315 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT38768 NZ_AP024568:359016-359315 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1CBF8