Detailed information of TA system TA038765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5465559..5466045
• Replicon: chromosome
• Accession: NZ_AP024565
• Organism: Microcystis aeruginosa strain NIES-88

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LC603172_RS28740
• Protein ID: WP_239698130.1
• Coordinates: 5465875..5466045 (-)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LC603172_RS27325
• Protein ID: WP_008202213.1
• Coordinates: 5465559..5465864 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC603172_RS27305 (MAN88_52940)	5461777..5463435	-	1659	WP_221275444.1	IS1634 family transposase	-
LC603172_RS27310	5463537..5463761	-	225	WP_260324483.1	DUF5615 family PIN-like protein	-
LC603172_RS27315 (MAN88_52950)	5463881..5465068	+	1188	WP_061432204.1	RNA-guided endonuclease TnpB family protein	-
LC603172_RS27320	5465322..5465468	+	147	WP_155726679.1	hypothetical protein	-
LC603172_RS27325 (MAN88_52960)	5465559..5465864	-	306	WP_008202213.1	HigA family addiction module antitoxin	Antitoxin
LC603172_RS28740	5465875..5466045	-	171	WP_239698130.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LC603172_RS27335 (MAN88_52970)	5466578..5467273	+	696	WP_260324484.1	PEP-CTERM sorting domain-containing protein	-
LC603172_RS27340 (MAN88_52980)	5467421..5467675	+	255	WP_061432203.1	hypothetical protein	-
LC603172_RS27345 (MAN88_52990)	5467672..5468127	+	456	WP_061432202.1	PIN domain-containing protein	-
LC603172_RS27350	5468355..5468498	-	144	WP_155726678.1	hypothetical protein	-
LC603172_RS27355 (MAN88_53000)	5468580..5468810	+	231	WP_061432201.1	addiction module protein	-
LC603172_RS27360 (MAN88_53010)	5469180..5469575	+	396	WP_221275445.1	hypothetical protein	-
LC603172_RS27365 (MAN88_53020)	5469484..5470470	-	987	WP_221275333.1	IS30-like element ISMae39 family transposase	-
LC603172_RS27370 (MAN88_53030)	5470681..5471034	+	354	WP_061432199.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	5461777..5476176	14399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-56)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-68)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6393.16 Da
• Isoelectric Point: 4.5813

>T38765 WP_239698130.1 NZ_AP024565:c5466045-5465875 [Microcystis aeruginosa]
MSAAESVEEANIPGYRLHPLTGELKGFWSVRVTGNWRIVFRFEDGNALDIDLVDYH

Nucleotide

• Length: 171 bp

>T38765 NZ_AP024565:c5466045-5465875 [Microcystis aeruginosa]
TTGTCGGCAGCGGAAAGTGTAGAGGAGGCTAATATCCCTGGTTACAGATTACACCCCCTGACGGGAGAGTTAAAAGGTTT
TTGGAGCGTCCGTGTTACAGGTAACTGGCGCATTGTCTTCCGATTTGAAGATGGAAACGCTCTCGACATTGACCTTGTAG
ATTATCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11168.11 Da
• Isoelectric Point: 10.7019

>AT38765 WP_008202213.1 NZ_AP024565:c5465864-5465559 [Microcystis aeruginosa]
MMTLKNPVHPGRIVKSSIEELELTVTAAAKMLNVSRSRLSNLINGKAGISPEMAVRLSKTIGSTPAFWLRLQMNYDLAEV
KKQEDRIIVNPIGLQHQTTLG

Nucleotide

• Length: 306 bp

>AT38765 NZ_AP024565:c5465864-5465559 [Microcystis aeruginosa]
ATGATGACCCTGAAAAATCCCGTACATCCGGGCAGAATTGTCAAAAGTTCAATTGAAGAGCTTGAATTGACCGTTACGGC
TGCGGCAAAAATGCTGAACGTGTCACGTTCTAGACTATCTAACCTAATCAATGGCAAGGCGGGGATTTCCCCGGAAATGG
CTGTGCGTTTGTCAAAGACAATCGGTAGCACCCCTGCTTTCTGGCTGCGCCTCCAGATGAATTACGATCTTGCTGAGGTT
AAAAAACAGGAAGATAGAATTATTGTGAATCCGATTGGGTTACAACACCAAACAACATTGGGTTAG