Detailed information of TA system TA038759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2545792..2546398
• Replicon: chromosome
• Accession: NZ_AP024565
• Organism: Microcystis aeruginosa strain NIES-88

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4I7E5
• Locus tag: LC603172_RS12660
• Protein ID: WP_002795678.1
• Coordinates: 2545792..2546073 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LC603172_RS12665
• Protein ID: WP_260324566.1
• Coordinates: 2546108..2546398 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC603172_RS12650 (MAN88_24530)	2541549..2544119	-	2571	WP_061432980.1	DUF3536 domain-containing protein	-
LC603172_RS28135	2544352..2544486	-	135	WP_260324565.1	hypothetical protein	-
LC603172_RS12655 (MAN88_24540)	2544543..2545082	-	540	WP_061432981.1	hypothetical protein	-
LC603172_RS12660 (MAN88_24550)	2545792..2546073	+	282	WP_002795678.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LC603172_RS12665 (MAN88_24560)	2546108..2546398	+	291	WP_260324566.1	HigA family addiction module antitoxin	Antitoxin
LC603172_RS12670	2546810..2547016	-	207	Protein_2547	Txe/YoeB family addiction module toxin	-
LC603172_RS12675 (MAN88_24570)	2547051..2547305	-	255	WP_061432983.1	hypothetical protein	-
LC603172_RS12680 (MAN88_24580)	2547372..2547542	+	171	WP_155726771.1	hypothetical protein	-
LC603172_RS28140	2547694..2547852	+	159	WP_239698191.1	hypothetical protein	-
LC603172_RS12690 (MAN88_24590)	2548215..2549474	-	1260	WP_061432984.1	glycosyltransferase family 4 protein	-
LC603172_RS12695 (MAN88_24600)	2549471..2550193	-	723	WP_061432985.1	FkbM family methyltransferase	-
LC603172_RS12700 (MAN88_24610)	2550251..2551075	-	825	WP_061432986.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-61)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10830.32 Da
• Isoelectric Point: 9.2038

>T38759 WP_002795678.1 NZ_AP024565:2545792-2546073 [Microcystis aeruginosa]
MIQSFRCKHTRLLFEGGDPPRFRGFKDTAIRKLTQLDAAQTLEFLRSPPGNQLEALKGDRKEQYSIRINSQFRLCFIWTN
NGPTDVEIVDYHS

Nucleotide

• Length: 282 bp

>T38759 NZ_AP024565:2545792-2546073 [Microcystis aeruginosa]
ATGATTCAATCTTTCCGATGCAAGCATACGCGATTACTTTTTGAAGGAGGAGATCCGCCTCGTTTTCGTGGTTTCAAAGA
TACTGCTATTCGGAAGTTGACTCAATTAGATGCGGCTCAAACCTTAGAGTTTCTGCGCTCTCCACCAGGCAATCAACTTG
AAGCTTTAAAAGGCGATAGAAAAGAACAATATAGTATTCGGATTAATTCTCAGTTTCGATTATGTTTTATCTGGACAAAT
AATGGACCTACTGATGTTGAAATTGTTGATTATCACTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10784.50 Da
• Isoelectric Point: 7.1655

>AT38759 WP_260324566.1 NZ_AP024565:2546108-2546398 [Microcystis aeruginosa]
MRPVHPGEILREDFLIPLNMSVNALALALKVPASRIHEIVKERRSISADTAARLACYFGGDAASWLSLQVIYDLKTLSNR
EEIIQQIQPRISEQIS

Nucleotide

• Length: 291 bp

>AT38759 NZ_AP024565:2546108-2546398 [Microcystis aeruginosa]
ATGCGACCCGTTCACCCAGGAGAAATTCTGCGGGAAGATTTTTTGATTCCTCTAAATATGAGCGTGAATGCTTTAGCTTT
AGCCTTGAAGGTTCCTGCATCTCGTATTCATGAAATTGTTAAAGAACGTCGTTCTATTTCGGCTGATACGGCAGCAAGAT
TAGCCTGCTATTTTGGCGGAGATGCTGCTTCCTGGCTCAGTCTTCAAGTAATTTATGATCTGAAGACTTTATCTAACCGT
GAGGAGATTATTCAGCAAATTCAGCCCCGTATTTCCGAACAAATTTCCTGA

Structures

Toxin

• AlphaFold DB: I4I7E5