Detailed information of TA system TA038735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1104800..1105527
• Replicon: chromosome
• Accession: NZ_AP024561
• Organism: Escherichia coli strain NIPH17_0036

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FLY99_RS05305
• Protein ID: WP_000547564.1
• Coordinates: 1105216..1105527 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FLY99_RS05300
• Protein ID: WP_000126294.1
• Coordinates: 1104800..1105219 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLY99_RS05280 (1099850)	1099850..1100377	-	528	WP_001078777.1	electron transport protein HydN	-
FLY99_RS05285 (1100526)	1100526..1101536	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FLY99_RS05290 (1101796)	1101796..1103253	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FLY99_RS05295 (1103262)	1103262..1104686	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FLY99_RS05300 (1104800)	1104800..1105219	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FLY99_RS05305 (1105216)	1105216..1105527	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FLY99_RS05310 (1105701)	1105701..1106462	-	762	WP_001026446.1	hypothetical protein	-
FLY99_RS05315 (1106487)	1106487..1106957	-	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
FLY99_RS05320 (1106950)	1106950..1107360	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FLY99_RS05325 (1107357)	1107357..1108124	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FLY99_RS05330 (1108124)	1108124..1108666	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FLY99_RS05335 (1108676)	1108676..1110385	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T38735 WP_000547564.1 NZ_AP024561:c1105527-1105216 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T38735 NZ_AP024561:c1105527-1105216 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT38735 WP_000126294.1 NZ_AP024561:c1105219-1104800 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT38735 NZ_AP024561:c1105219-1104800 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5