Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2129016..2129743 Replicon chromosome
Accession NZ_AP024560
Organism Escherichia coli strain NIPH17_0020

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag FLY91_RS10225 Protein ID WP_000547564.1
Coordinates 2129016..2129327 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FLY91_RS10230 Protein ID WP_000126294.1
Coordinates 2129324..2129743 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY91_RS10195 (2124158) 2124158..2125867 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
FLY91_RS10200 (2125877) 2125877..2126419 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
FLY91_RS10205 (2126419) 2126419..2127186 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
FLY91_RS10210 (2127183) 2127183..2127593 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
FLY91_RS10215 (2127586) 2127586..2128056 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
FLY91_RS10220 (2128081) 2128081..2128842 + 762 WP_001026446.1 hypothetical protein -
FLY91_RS10225 (2129016) 2129016..2129327 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
FLY91_RS10230 (2129324) 2129324..2129743 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
FLY91_RS10235 (2129857) 2129857..2131281 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
FLY91_RS10240 (2131290) 2131290..2132747 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
FLY91_RS10245 (2133007) 2133007..2134017 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
FLY91_RS10250 (2134166) 2134166..2134693 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2081779..2130177 48398


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T38709 WP_000547564.1 NZ_AP024560:2129016-2129327 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T38709 NZ_AP024560:2129016-2129327 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT38709 WP_000126294.1 NZ_AP024560:2129324-2129743 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT38709 NZ_AP024560:2129324-2129743 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5


Antitoxin

Source ID Structure

References