Detailed information of TA system TA038693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 46303..46918
• Replicon: chromosome
• Accession: NZ_AP024556
• Organism: Vibrio cholerae strain IDH-03506

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: K6J79_RS14200
• Protein ID: WP_001162670.1
• Coordinates: 46303..46590 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J79_RS14205
• Protein ID: WP_001232701.1
• Coordinates: 46601..46918 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J79_RS18950	41646..41821	+	176	Protein_87	acetyltransferase	-
K6J79_RS18955	41882..42034	+	153	Protein_88	DUF645 family protein	-
K6J79_RS14165	42228..42545	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K6J79_RS14170	42564..42806	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K6J79_RS14175	43048..43557	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
K6J79_RS14180	43614..44267	+	654	WP_000226874.1	hypothetical protein	-
K6J79_RS18960	44356..44565	+	210	WP_001900246.1	DUF3709 domain-containing protein	-
K6J79_RS14185	44577..44795	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
K6J79_RS18965	44984..45177	+	194	Protein_95	hypothetical protein	-
K6J79_RS14190	45198..45431	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
K6J79_RS14195	45856..46036	+	181	Protein_97	DUF645 family protein	-
K6J79_RS18970	46149..46262	+	114	WP_001894955.1	hypothetical protein	-
K6J79_RS14200	46303..46590	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J79_RS14205	46601..46918	+	318	WP_001232701.1	HigA family addiction module antitoxin	Antitoxin
K6J79_RS18975	46915..47025	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
K6J79_RS18980	47064..47174	+	111	Protein_102	acetyltransferase	-
K6J79_RS14210	47202..47327	+	126	WP_001944767.1	DUF645 family protein	-
K6J79_RS18985	47290..47406	+	117	WP_001889170.1	hypothetical protein	-
K6J79_RS14220	47594..48598	+	1005	WP_000964925.1	LD-carboxypeptidase	-
K6J79_RS14225	48751..49197	+	447	WP_000128977.1	hypothetical protein	-
K6J79_RS14230	49317..50246	+	930	WP_000335802.1	hypothetical protein	-
K6J79_RS18990	50243..50353	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
K6J79_RS14235	50381..50857	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
K6J79_RS14240	51048..51509	+	462	WP_001911752.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	cqsA / ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA	3..1061958	1061955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T38693 WP_001162670.1 NZ_AP024556:46303-46590 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T38693 NZ_AP024556:46303-46590 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT38693 WP_001232701.1 NZ_AP024556:46601-46918 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT38693 NZ_AP024556:46601-46918 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36