Detailed information of TA system TA038640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 845920..846535
• Replicon: chromosome
• Accession: NZ_AP024550
• Organism: Vibrio cholerae strain IDH-00113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: K6J78_RS17460
• Protein ID: WP_001162670.1
• Coordinates: 845920..846207 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J78_RS17465
• Protein ID: WP_001232701.1
• Coordinates: 846218..846535 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J78_RS18755	841262..841437	+	176	Protein_778	acetyltransferase	-
K6J78_RS18760	841498..841650	+	153	Protein_779	DUF645 family protein	-
K6J78_RS17425	841844..842161	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K6J78_RS17430	842180..842423	-	244	Protein_781	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K6J78_RS17435	842665..843174	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
K6J78_RS17440	843231..843884	+	654	WP_000226874.1	hypothetical protein	-
K6J78_RS18765	843973..844182	+	210	WP_001900246.1	DUF3709 domain-containing protein	-
K6J78_RS17445	844194..844412	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
K6J78_RS18770	844601..844794	+	194	Protein_786	hypothetical protein	-
K6J78_RS17450	844815..845048	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
K6J78_RS17455	845473..845653	+	181	Protein_788	DUF645 family protein	-
K6J78_RS18775	845766..845879	+	114	WP_001894955.1	hypothetical protein	-
K6J78_RS17460	845920..846207	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J78_RS17465	846218..846535	+	318	WP_001232701.1	HigA family addiction module antitoxin	Antitoxin
K6J78_RS18780	846532..846642	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
K6J78_RS18785	846681..846791	+	111	Protein_793	acetyltransferase	-
K6J78_RS17470	846819..846944	+	126	WP_001944767.1	DUF645 family protein	-
K6J78_RS18790	846907..847023	+	117	WP_001889170.1	hypothetical protein	-
K6J78_RS17480	847211..848215	+	1005	WP_000964925.1	LD-carboxypeptidase	-
K6J78_RS17485	848368..848814	+	447	WP_000128977.1	hypothetical protein	-
K6J78_RS17490	848934..849863	+	930	WP_000335802.1	hypothetical protein	-
K6J78_RS18795	849860..849970	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
K6J78_RS17495	849998..850474	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
K6J78_RS17500	850665..851126	+	462	WP_001911752.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	782725..888585	105860
	-	inside	Integron	-	-	835106..888236	53130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T38640 WP_001162670.1 NZ_AP024550:845920-846207 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T38640 NZ_AP024550:845920-846207 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT38640 WP_001232701.1 NZ_AP024550:846218-846535 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT38640 NZ_AP024550:846218-846535 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36