Detailed information of TA system TA038603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4345727..4346329
• Replicon: chromosome
• Accession: NZ_AP024521
• Organism: Escherichia coli strain E189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LDO00_RS21090
• Protein ID: WP_000897305.1
• Coordinates: 4345727..4346038 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO00_RS21095
• Protein ID: WP_000356397.1
• Coordinates: 4346039..4346329 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO00_RS21060 (4340757)	4340757..4341542	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
LDO00_RS21065 (4341641)	4341641..4342240	+	600	WP_001295269.1	glucose-1-phosphatase	-
LDO00_RS21070 (4342234)	4342234..4343106	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LDO00_RS21075 (4343103)	4343103..4343540	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LDO00_RS21080 (4343585)	4343585..4344526	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LDO00_RS21085 (4344590)	4344590..4345498	-	909	WP_001162704.1	alpha/beta hydrolase	-
LDO00_RS21090 (4345727)	4345727..4346038	+	312	WP_000897305.1	hypothetical protein	Toxin
LDO00_RS21095 (4346039)	4346039..4346329	+	291	WP_000356397.1	NadS family protein	Antitoxin
LDO00_RS21100 (4346915)	4346915..4347133	+	219	WP_001295676.1	CopG family transcriptional regulator	-
LDO00_RS21105 (4347352)	4347352..4347594	+	243	WP_001086388.1	protein YiiF	-
LDO00_RS21110 (4347924)	4347924..4348853	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LDO00_RS21115 (4348850)	4348850..4349485	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LDO00_RS21120 (4349482)	4349482..4350384	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T38603 WP_000897305.1 NZ_AP024521:4345727-4346038 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T38603 NZ_AP024521:4345727-4346038 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT38603 WP_000356397.1 NZ_AP024521:4346039-4346329 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT38603 NZ_AP024521:4346039-4346329 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7