Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 281125..281666 Replicon chromosome
Accession NZ_AP024521
Organism Escherichia coli strain E189

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LDO00_RS01275 Protein ID WP_000615976.1
Coordinates 281125..281403 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LDO00_RS01280 Protein ID WP_000729704.1
Coordinates 281406..281666 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO00_RS01260 (278707) 278707..279285 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LDO00_RS01265 (279491) 279491..280258 + 768 WP_000333380.1 class II glutamine amidotransferase -
LDO00_RS01270 (280229) 280229..280969 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LDO00_RS01275 (281125) 281125..281403 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LDO00_RS01280 (281406) 281406..281666 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LDO00_RS01285 (281852) 281852..282625 + 774 WP_000543899.1 C40 family peptidase -
LDO00_RS01290 (282682) 282682..283038 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LDO00_RS01295 (283031) 283031..283309 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LDO00_RS01300 (283414) 283414..285507 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
LDO00_RS01305 (285491) 285491..286630 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T38582 WP_000615976.1 NZ_AP024521:c281403-281125 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T38582 NZ_AP024521:c281403-281125 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT38582 WP_000729704.1 NZ_AP024521:c281666-281406 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT38582 NZ_AP024521:c281666-281406 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References