Detailed information of TA system TA038578

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97517..97943
• Replicon: plasmid pE148-1
• Accession: NZ_AP024519
• Organism: Escherichia coli strain E148

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LDN98_RS24815
• Protein ID: WP_001372321.1
• Coordinates: 97517..97642 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	97719..97943 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN98_RS24775 (92568)	92568..92795	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
LDN98_RS24780 (92889)	92889..93575	-	687	WP_000332484.1	PAS domain-containing protein	-
LDN98_RS24785 (93766)	93766..94149	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LDN98_RS24790 (94426)	94426..95073	+	648	WP_000614934.1	transglycosylase SLT domain-containing protein	-
LDN98_RS24795 (95370)	95370..96191	-	822	WP_021573168.1	DUF932 domain-containing protein	-
LDN98_RS24800 (96308)	96308..96595	-	288	WP_000107538.1	hypothetical protein	-
LDN98_RS24805 (96896)	96896..97057	+	162	Protein_117	hypothetical protein	-
LDN98_RS24810 (97067)	97067..97297	-	231	WP_223951682.1	hypothetical protein	-
LDN98_RS24815 (97517)	97517..97642	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LDN98_RS24820 (97584)	97584..97733	-	150	Protein_120	plasmid maintenance protein Mok	-
- (97719)	97719..97943	-	225	NuclAT_0	-	Antitoxin
- (97719)	97719..97943	-	225	NuclAT_0	-	Antitoxin
- (97719)	97719..97943	-	225	NuclAT_0	-	Antitoxin
- (97719)	97719..97943	-	225	NuclAT_0	-	Antitoxin
LDN98_RS24825 (97912)	97912..98674	-	763	Protein_121	plasmid SOS inhibition protein A	-
LDN98_RS24830 (98671)	98671..99105	-	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / tet(A) / mph(A)	-	1..100617	100617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T38578 WP_001372321.1 NZ_AP024519:c97642-97517 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T38578 NZ_AP024519:c97642-97517 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT38578 NZ_AP024519:c97943-97719 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin