Detailed information of TA system TA038573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4365906..4366508
• Replicon: chromosome
• Accession: NZ_AP024518
• Organism: Escherichia coli strain E148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LDN98_RS21270
• Protein ID: WP_000897305.1
• Coordinates: 4365906..4366217 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDN98_RS21275
• Protein ID: WP_000356397.1
• Coordinates: 4366218..4366508 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN98_RS21240 (4360936)	4360936..4361721	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
LDN98_RS21245 (4361820)	4361820..4362419	+	600	WP_001295269.1	glucose-1-phosphatase	-
LDN98_RS21250 (4362413)	4362413..4363285	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LDN98_RS21255 (4363282)	4363282..4363719	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LDN98_RS21260 (4363764)	4363764..4364705	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LDN98_RS21265 (4364769)	4364769..4365677	-	909	WP_001162704.1	alpha/beta hydrolase	-
LDN98_RS21270 (4365906)	4365906..4366217	+	312	WP_000897305.1	hypothetical protein	Toxin
LDN98_RS21275 (4366218)	4366218..4366508	+	291	WP_000356397.1	NadS family protein	Antitoxin
LDN98_RS21280 (4367094)	4367094..4367312	+	219	WP_001295676.1	CopG family transcriptional regulator	-
LDN98_RS21285 (4367531)	4367531..4367773	+	243	WP_001086388.1	protein YiiF	-
LDN98_RS21290 (4368103)	4368103..4369032	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LDN98_RS21295 (4369029)	4369029..4369664	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LDN98_RS21300 (4369661)	4369661..4370563	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T38573 WP_000897305.1 NZ_AP024518:4365906-4366217 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T38573 NZ_AP024518:4365906-4366217 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT38573 WP_000356397.1 NZ_AP024518:4366218-4366508 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT38573 NZ_AP024518:4366218-4366508 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7