Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 575439..576118 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag LDN98_RS02760 Protein ID WP_000057540.1
Coordinates 575439..575741 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LDN98_RS02765 Protein ID WP_000806442.1
Coordinates 575777..576118 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS02740 (570702) 570702..571922 - 1221 WP_001251608.1 fosmidomycin MFS transporter -
LDN98_RS02745 (572140) 572140..573792 + 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LDN98_RS02750 (573829) 573829..574308 - 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LDN98_RS02755 (574512) 574512..575306 - 795 WP_000365180.1 TraB/GumN family protein -
LDN98_RS02760 (575439) 575439..575741 + 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LDN98_RS02765 (575777) 575777..576118 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LDN98_RS02770 (576176) 576176..578680 - 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
LDN98_RS02775 (578942) 578942..579874 + 933 WP_023155692.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T38554 WP_000057540.1 NZ_AP024518:575439-575741 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T38554 NZ_AP024518:575439-575741 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCTCGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT38554 WP_000806442.1 NZ_AP024518:575777-576118 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT38554 NZ_AP024518:575777-576118 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References