Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 281126..281667 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LDN98_RS01275 Protein ID WP_000615976.1
Coordinates 281126..281404 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LDN98_RS01280 Protein ID WP_000729704.1
Coordinates 281407..281667 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS01260 (278708) 278708..279286 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LDN98_RS01265 (279492) 279492..280259 + 768 WP_000333380.1 class II glutamine amidotransferase -
LDN98_RS01270 (280230) 280230..280970 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LDN98_RS01275 (281126) 281126..281404 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LDN98_RS01280 (281407) 281407..281667 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LDN98_RS01285 (281853) 281853..282626 + 774 WP_000543899.1 C40 family peptidase -
LDN98_RS01290 (282683) 282683..283039 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LDN98_RS01295 (283032) 283032..283310 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LDN98_RS01300 (283415) 283415..285508 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
LDN98_RS01305 (285492) 285492..286631 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T38552 WP_000615976.1 NZ_AP024518:c281404-281126 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T38552 NZ_AP024518:c281404-281126 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT38552 WP_000729704.1 NZ_AP024518:c281667-281407 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT38552 NZ_AP024518:c281667-281407 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References